Adaptive Biochemistry Laboratory, Pós-Graduate Program in Toxicological Biochemistry, Department of Chemistry, Federal University of Santa Maria (UFSM), Santa Maria, 97105.900 RS, Brazil.
Department of Plant Science, Federal University of Santa Maria, RS, Brazil.
Ecotoxicol Environ Saf. 2014 Mar;101:77-82. doi: 10.1016/j.ecoenv.2013.12.012. Epub 2014 Jan 11.
Effects of carbofuran commercial formulation on oxidative stress parameters were studied in carps (Cyprinus carpio) exposed to 50µg/L for 7 and 30 days under rice field and laboratory conditions. Thiobarbituric acid reactive substance (TBARS) levels were increased in the brain of fish after 7 and 30 days under rice field and laboratory conditions. In the liver and muscle, TBARS levels increased after 7 and 30 days under laboratory conditions, whereas in rice field the levels increased only after 30 days. Protein carbonyl content in the liver increased after 7 and 30 days under both experimental conditions. Acetylcholinesterase (AChE) activity was decreased in the brain and muscle after 7 and 30 days under both experimental conditions evaluated. The superoxide dismutase (SOD) activity increased in the liver after 7 and 30 days under rice field condition, whereas under laboratory condition this enzyme increased only after 30 days. The catalase (CAT) activity in the liver decreased after 30 days under rice field condition, whereas no changes were observed under laboratory conditions. In rice field, glutathione S-transferase (GST) decreased after 7 days but increased after 30 days, whereas no change was observed in fish exposed to carbofuran under laboratory conditions. These results suggest that environmental relevant carbofuran concentrations may cause oxidative stress, affecting biochemical and enzymatic parameters on carps. Some parameters could be used as biomarkers to carbofuran exposure.
在稻田和实验室条件下，将鲤鱼（Cyprinus carpio）暴露于 50µg/L 下 7 天和 30 天，研究了呋喃丹商业制剂对氧化应激参数的影响。在 7 天和 30 天的稻田和实验室条件下，鱼脑中的硫代巴比妥酸反应物质（TBARS）水平升高。在肝脏和肌肉中，TBARS 水平在实验室条件下 7 天和 30 天后均升高，而在稻田中仅在 30 天后升高。在两种实验条件下，肝脏中的蛋白质羰基含量在 7 天和 30 天后均增加。在两种实验条件下，7 天和 30 天后，鱼脑和肌肉中的乙酰胆碱酯酶（AChE）活性均降低。在稻田条件下，7 天和 30 天后肝脏中超氧化物歧化酶（SOD）活性增加，而在实验室条件下，该酶仅在 30 天后增加。在稻田条件下，肝脏中的过氧化氢酶（CAT）活性在 30 天后降低，而在实验室条件下没有观察到变化。在稻田中，谷胱甘肽 S-转移酶（GST）在 7 天降低，但在 30 天后升高，而在实验室条件下暴露于呋喃丹的鱼中未观察到变化。这些结果表明，环境相关浓度的呋喃丹可能导致氧化应激，影响鲤鱼的生化和酶学参数。一些参数可作为呋喃丹暴露的生物标志物。
