Adaptive Laboratory of Biochemistry, Post-Graduation Program in Biological Science-Toxicologic Biochemistry, Department of Chemistry, University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.
Ecotoxicol Environ Saf. 2013 Jun;92:27-31. doi: 10.1016/j.ecoenv.2013.01.028. Epub 2013 Mar 7.
This study evaluated parameters of oxidative stress and antioxidant profile in fish after herbicide exposure. Cyprinus carpio were exposed to quinclorac (initial concentration 344.60μg/L) for 7, 30, and 90 days under rice field condition. Thiobarbituric acid-reactive substances (TBARS) were evaluated in brain, liver and muscle tissues, and protein carbonyl in liver. Enzymatic parameters such as catalase (CAT) and gluthatione S-transferase (GST) activities also were studied in liver. TBARS levels fluctuated in the brain showing increase in 7 days and decrease in 30 days, while in liver it was observed increase in 7 and 30 days, as well as in muscle after 30 and 90 days. The protein carbonyl was also increased after 30 and 90 days of herbicide exposure. CAT and GST activities were decreased after 30 and 90 days, respectively. The alterations observed suggest that a commercial formulation containing quinclorac causes oxidative damage in different tissues of carp after a long time of exposure. This study pointed out the importance of quinclorac toxicity considering the concentration used in rice fields.
本研究评估了鱼类在接触除草剂后的氧化应激和抗氧化特性参数。鲤鱼在稻田条件下暴露于百草枯（初始浓度 344.60μg/L）7、30 和 90 天。在脑、肝和肌肉组织中评估了硫代巴比妥酸反应物质（TBARS），在肝中还研究了酶参数如过氧化氢酶（CAT）和谷胱甘肽 S-转移酶（GST）的活性。大脑中的 TBARS 水平波动，7 天增加，30 天减少，而肝脏中则在 7 和 30 天增加，肌肉中在 30 和 90 天后也增加。在接触除草剂 30 和 90 天后，蛋白质羰基也增加。CAT 和 GST 活性分别在 30 和 90 天后降低。这些观察到的改变表明，含有百草枯的商业制剂在长时间暴露后会对鲤鱼的不同组织造成氧化损伤。考虑到稻田中使用的浓度，本研究指出了百草枯毒性的重要性。
