Fehér E, Pénzes L
First Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Gerontology. 1987;33(6):341-8. doi: 10.1159/000212901.
Immunoelectron microscopic investigations were carried out to study the substance P, vasoactive intestinal polypeptide (VIP) and somatostatin immunoreactive nerve elements in the wall of the small intestine. In young and old animals a large number of immunoreactive nerve fibers were found in all layers of the small intestine. They were observed closely to the epithelial cells, to the blood vessel basement membrane and to the smooth muscle cells and in some cases they were observed in a synapse with other unlabelled nerve fibers. On the other hand, in the senile animals very few immunoreactive nerve fibers were observed, calculated for a 100 micron2 tissue area. In the senile animals the overall number of nerve fibers was decreased in comparison to the young and old animals and most of them were in degeneration. This change could be the cause of the changes in the senescence-related epithelial transport processes and furthermore, of the modifications of the overall intestinal motility of the gastrointestinal tract, resulting in the age-dependent transit rates.
进行免疫电子显微镜研究以探讨小肠壁中P物质、血管活性肠肽（VIP）和生长抑素免疫反应性神经元件。在幼年和老年动物中，小肠各层均发现大量免疫反应性神经纤维。它们靠近上皮细胞、血管基底膜和平滑肌细胞，在某些情况下，还观察到它们与其他未标记的神经纤维形成突触。另一方面，在老年动物中，按100平方微米组织面积计算，观察到的免疫反应性神经纤维极少。与幼年和成年动物相比，老年动物神经纤维总数减少，且大多数处于退化状态。这种变化可能是衰老相关上皮运输过程变化的原因，进而也是胃肠道整体肠道运动改变的原因，导致了与年龄相关的转运速率。
