Accili E A, Dhatt N, Buchan A M
Department of Physiology, University of British Columbia, Vancouver, Canada.
Neurosci Lett. 1995 Feb 6;185(1):37-40. doi: 10.1016/0304-3940(94)11219-9.
The distribution and co-localization of substance P immunoreactivity (SP-IR), somatostatin-IR (SS-IR) and vasoactive intestinal peptide-IR (VIP-IR) have been determined in canine and human jejunum by immunocytochemistry (ICC). Immunostaining with an antibody to protein gene product 9.5 revealed fewer neuronal cell bodies in the submucosal ganglion of human than in canine intestine. Double immunostaining demonstrated that SP-IR and SS-IR were always co-localized in human but never in canine submucosal neurons. In both species, VIP-IR was present in a separate population of neurons. In canine submucosal ganglia, each peptide represented approximately one-third of the neurons while the proportions of VIP and SP/SS-containing neurons in human were 40% and 42%, respectively. These results provide neuroanatomical evidence for different functions of neural SP and SS in canine and human jejunum.
通过免疫细胞化学（ICC）方法，已确定了P物质免疫反应性（SP-IR）、生长抑素免疫反应性（SS-IR）和血管活性肠肽免疫反应性（VIP-IR）在犬和人空肠中的分布及共定位情况。用蛋白基因产物9.5抗体进行免疫染色显示，人空肠黏膜下神经节中的神经元细胞体比犬空肠中的少。双重免疫染色表明，SP-IR和SS-IR在人空肠黏膜下神经元中总是共定位，而在犬空肠中则从未共定位。在这两个物种中，VIP-IR存在于单独的神经元群体中。在犬空肠黏膜下神经节中，每种肽大约占神经元的三分之一，而在人空肠中，含VIP和含SP/SS的神经元比例分别为40%和42%。这些结果为神经SP和SS在犬和人空肠中的不同功能提供了神经解剖学证据。
