Laboratory of Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
BK 21 FOUR Program for Future Veterinary Medicine Leading Education and Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
J Vet Intern Med. 2023 Mar;37(2):484-489. doi: 10.1111/jvim.16641. Epub 2023 Feb 10.
Evaluating regeneration is essential for the classification and differential diagnosis of anemia in dogs. Early detection of regeneration is challenging in anemic dogs.
This study assessed the value of immature reticulocyte fraction (IRF) in differentiating preregenerative anemia (PRA) from nonregenerative anemia (NRA) in dogs.
Ninety-four dogs: 49 controls and 45 with anemia.
Case-control study. Fractions of low-, medium- (MFR), and high-fluorescence reticulocytes (HFR), were measured using the ADVIA 2120i hematology analyzer. The IRF was calculated as the sum of percentages of MFR and HFR. Data from dogs with regenerative anemia (RA, n = 19), PRA (n = 11), and NRA (n = 15) were retrospectively analyzed. The value of IRF was compared with reticulocyte production index (RPI) using the receiver operating characteristic (ROC) curve.
The median of IRF was significantly higher in dogs with RA (46.5%; range, 40.9-53.6%; P < .001) and PRA (40.6%; range, 27.7-47.1%; P = .01) than in controls (22.1%; range, 16.9-29.3%). The IRF in dogs with PRA showed no difference compared to dogs with RA (P > .99) but was higher than dogs with NRA (18.7%; range, 8.8-24%; P = .00). The area under the ROC curve of IRF was superior to that of RPI (0.897 vs 0.818, P = .00) in differentiating dogs with PRA from NRA.
The IRF is a reliable variable for detecting early regeneration in anemic dogs without reticulocytosis. The study suggests that the measurement of IRF could be useful in classifying anemic dogs.
评估再生对于犬贫血的分类和鉴别诊断至关重要。在贫血犬中，早期发现再生具有挑战性。
本研究评估未成熟网织红细胞分数（IRF）在区分前再生性贫血（PRA）和非再生性贫血（NRA）犬中的价值。
94 只狗：49 只对照犬和 45 只贫血犬。
病例对照研究。使用 ADVIA 2120i 血液分析仪测量低、中（MFR）和高荧光网织红细胞（HFR）的分数。IRF 计算为 MFR 和 HFR 百分比的总和。回顾性分析了 19 只再生性贫血（RA）、11 只 PRA 和 15 只 NRA 犬的数据。使用受试者工作特征（ROC）曲线比较 IRF 与网织红细胞生成指数（RPI）。
RA（46.5%；范围，40.9-53.6%；P < .001）和 PRA（40.6%；范围，27.7-47.1%；P = .01）犬的 IRF 中位数显著高于对照犬（22.1%；范围，16.9-29.3%）。PRA 犬的 IRF 与 RA 犬无差异（P > .99），但高于 NRA 犬（18.7%；范围，8.8-24%；P = .00）。IRF 的 ROC 曲线下面积优于 RPI（0.897 比 0.818，P = .00），可用于区分 PRA 和 NRA 犬。
IRF 是一种可靠的变量，可用于检测无网织红细胞增多的贫血犬的早期再生。该研究表明，IRF 测量可用于对贫血犬进行分类。
