Andersen A B, Worsaae A, Chaparas S D
Laboratory of Mycobacteria and Cellular Immunology, Food and Drug Administration, Bethesda, Maryland 20892.
Infect Immun. 1988 May;56(5):1344-51. doi: 10.1128/iai.56.5.1344-1351.1988.
A genomic lambda gt11 DNA library of Mycobacterium tuberculosis was screened for expression of mycobacterial protein antigens with murine monoclonal antibodies. The reactivity patterns of the monoclonal antibodies ranged from those showing a limited interspecies reactivity to antibodies widely cross-reactive among different mycobacterial species. Twelve recombinant bacteriophages were isolated, containing eight mycobacterial genes (paa, pab, pac, pad, paeA, paeB, pafA, and pafB) encoding protein antigens. Physical maps of the phages were generated and the products of the recombinant genes were analyzed by immunoblotting techniques. PaeA and PaeB are distinct proteins but were shown to share an epitope. A similar condition was observed between PafA and PafB. Among the phages isolated, two groups expressed epitopes specific for M. tuberculosis and Mycobacterium bovis BCG. One group of phages produced an antigenic determinant which is found in M. tuberculosis and Mycobacterium marinum but not in M. bovis BCG.
利用鼠单克隆抗体筛选结核分枝杆菌的基因组λgt11 DNA文库，以检测分枝杆菌蛋白抗原的表达。单克隆抗体的反应模式各不相同，从显示有限种间反应性的抗体到在不同分枝杆菌物种间广泛交叉反应的抗体。分离出12个重组噬菌体，它们包含8个编码蛋白抗原的分枝杆菌基因（paa、pab、pac、pad、paeA、paeB、pafA和pafB）。构建了噬菌体的物理图谱，并通过免疫印迹技术分析了重组基因的产物。PaeA和PaeB是不同的蛋白质，但显示有一个共同表位。PafA和PafB之间也观察到类似情况。在分离出的噬菌体中，有两组表达了对结核分枝杆菌和牛分枝杆菌卡介苗特异的表位。一组噬菌体产生了一种抗原决定簇，该决定簇存在于结核分枝杆菌和海分枝杆菌中，但不存在于牛分枝杆菌卡介苗中。
