Saxena Manjusha, Mir Abrar Hussain, Sharma Manik, Malla Mohd Yousu, Qureshi Sumeerah, Mir Mohd Iqbal, Chaturvedi Yogesh
P.G. Department of Botany, Govt. Maharaja Autonomous P.G. College, Chhatarpur-471405, M.P, India.
Pest Control and Ayurvedic Drug Research Laboratory, S.S.L. Jain P.G. College, Vidisha-464001, M.P. India.
Pak J Biol Sci. 2013 Dec 15;16(24):1971-7. doi: 10.3923/pjbs.2013.1971.1977.
Tridax procumbens L., Asteraceae, has been extensively used for various ailments in the Ayurvedic system of medicine. Previous studies have revealed remarkable phytoconstituents from Tridax procumbens L. with significant antioxidant activity. The aim of the present study is to measure the anti-DPPH activity of the purified isolated compounds from n butanol soluble part and ethyl acetate soluble part of successive methanolic extract of Tridax procumbens L. We thus quantified the total phenolic and total flavonoids in different purified isolated compounds, the whole of the tests were evaluated with a sample cone. of 100 microg mL(-1) and were determined spectrophotometrically using Folin-ciocaltue and AlCl3 reagents, respectively. DPPH (1,1-diphenyl, 2-picryl hydrazyl) assay was used to determine the in vitro antioxidant activity of different isolated compounds. Isolated compounds, one from ethyl acetate soluble part (EF-I) and one from n butanol soluble part (BF-II) were reported to possess a significant anti DPPH activity with lowest IC50 values 67.26 and 80.90 microg mL(-1), respectively while comparable to standard ascorbic acid with IC50 value of 59.62 microg mL(-1), due to the high concentration of phenols 146.4 microg mL(-1) from EF-I and 142.2 microg mL(-1) from BF-II and flavonoids 48 and 42.5 microg mL(-1) found in EF-I and BF-II isolated compounds, respectively.
三叶鬼针草，菊科，在阿育吠陀医学体系中已被广泛用于治疗各种疾病。先前的研究已经揭示了三叶鬼针草中具有显著抗氧化活性的重要植物成分。本研究的目的是测量从三叶鬼针草连续甲醇提取物的正丁醇可溶部分和乙酸乙酯可溶部分中纯化分离出的化合物的抗DPPH活性。因此，我们对不同纯化分离化合物中的总酚和总黄酮进行了定量，所有测试均使用100微克/毫升的样品浓度进行评估，并分别使用福林-西奥卡勒试剂和氯化铝试剂通过分光光度法进行测定。DPPH（1,1-二苯基，2-苦基肼）测定法用于测定不同分离化合物的体外抗氧化活性。据报道，从乙酸乙酯可溶部分分离出的一种化合物（EF-I）和从正丁醇可溶部分分离出的一种化合物（BF-II）具有显著的抗DPPH活性，最低IC50值分别为67.26和80.90微克/毫升，与IC50值为59.62微克/毫升的标准抗坏血酸相当，这是由于在EF-I分离化合物中发现了高浓度的酚（146.4微克/毫升）和黄酮（48微克/毫升），在BF-II分离化合物中发现了高浓度的酚（142.2微克/毫升）和黄酮（42.5微克/毫升）。
