傅里叶变换红外光谱法：一种用于无创检测精子发生的潜在技术。
Fourier transform infrared spectroscopy: a potential technique for noninvasive detection of spermatogenesis.
作者信息
Gilany Kambiz, Pouracil Roudabeh Sadat Moazeni, Sadeghi Mohammad Reza
机构信息
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Department of Chemistry, Sharif University of Technology, Tehran, Iran.
出版信息
Avicenna J Med Biotechnol. 2014 Jan;6(1):47-52.
BACKGROUND
The seminal plasma is an excellent source for noninvasive detection of spermatogenesis. The seminal plasma of normospermic and azoospermic men has been analyzed for detection of spermatogenesis.
METHODS
Optical spectroscopy (Attenuated Total Reflectance-Infrared spectroscopy (ATR-IR) and Fourier Transform infrared spectroscopy (FT-IR) has been used to analyze the seminal plasma and the metabolome of seminal plasma for detection of spermatogenesis.
RESULTS
The seminal plasma of normospermic and azoospermic men has been analyzed by ATR-IR. The results show that there is a pattern variation in the azoospermic men compared to normospermic men. However, the seminal plasma is too complex to show significant pattern variation. Therefore, the metabolome which is a subcomponent of the seminal plasma was analyzed. The seminal plasma metabolome of normospermic and azoospermic men has been analyzed by FT-IR. A significant pattern change was observed. The data combined with chemometrics analysis showed that significant changes are observed at metabolome level.
CONCLUSION
We suggest that FT-IR has the potential as a diagnostic tool instead of testicular biopsy.
背景
精浆是无创检测精子发生的理想来源。已对正常精子症和无精子症男性的精浆进行分析以检测精子发生。
方法
利用光谱学（衰减全反射红外光谱法（ATR-IR）和傅里叶变换红外光谱法（FT-IR））分析精浆及其代谢组以检测精子发生。
结果
已通过ATR-IR对正常精子症和无精子症男性的精浆进行分析。结果表明，与正常精子症男性相比，无精子症男性存在模式变化。然而，精浆过于复杂，无法显示出明显的模式变化。因此，对作为精浆子成分的代谢组进行了分析。已通过FT-IR对正常精子症和无精子症男性的精浆代谢组进行分析。观察到显著的模式变化。结合化学计量学分析的数据表明，在代谢组水平观察到显著变化。
结论
我们认为FT-IR有潜力作为一种诊断工具，取代睾丸活检。
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