Balavandy Sepideh Keshan, Shameli Kamyar, Biak Dayang Radiah Binti Awang, Abidin Zurina Zainal
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Chem Cent J. 2014 Feb 13;8(1):11. doi: 10.1186/1752-153X-8-11.
This study aims to investigate the influence of different stirring time for synthesis of silver nanoparticles in glutathione (GSH) aqueous solution. The silver nanoparticles (Ag-NPs) were prepared by green synthesis method using GSH as reducing agent and stabilizer, under moderate temperature at different stirring times. Silver nitrate (AgNO3) was taken as the metal precursor while Ag-NPs were prepared in the over reaction time.
Formation of Ag-NPs was determined by UV-vis spectroscopy where surface plasmon absorption maxima can be observed at 344-354 nm from the UV-vis spectrum. The synthesized nanoparticles were also characterized by X-ray diffraction (XRD). The peaks in the XRD pattern confirmed that the Ag-NPs possessed a face-centered cubic and peaks of contaminated crystalline phases were unable to be located. Transmission electron microscopy (TEM) revealed that Ag-NPs synthesized were in spherical shape. Zeta potential results indicate that the stability of the Ag-NPs is increases at the 72 h stirring time of reaction comparison to GSH. The Fourier transform infrared (FT-IR) spectrum suggested the complexation present between GSH and Ag-NPs. The use of green chemistry reagents, such as peptide, provides green and economic features to this work.
Ag-NPs were successfully synthesized in GSH aqueous solution under moderate temperature at different stirring times. The study clearly showed that the Ag-NPs synthesized in the long times of stirring, thus, the kinetic of GSH reaction is very slow. TEM results shows that with the increase of stirring times the mean particle size of Ag-NPs become increases. The FT-IR spectrum suggested the complexation present between GSH and Ag-NPs. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field.
本研究旨在探究谷胱甘肽（GSH）水溶液中不同搅拌时间对银纳米颗粒合成的影响。以GSH作为还原剂和稳定剂，在不同搅拌时间的适中温度下，通过绿色合成法制备银纳米颗粒（Ag-NPs）。以硝酸银（AgNO₃）作为金属前驱体，在过量反应时间内制备Ag-NPs。
通过紫外可见光谱法确定Ag-NPs的形成，从紫外可见光谱中可观察到表面等离子体吸收最大值在344 - 354 nm处。合成的纳米颗粒还通过X射线衍射（XRD）进行了表征。XRD图谱中的峰证实Ag-NPs具有面心立方结构，且未发现污染晶相的峰。透射电子显微镜（TEM）显示合成的Ag-NPs呈球形。zeta电位结果表明，与GSH相比，在反应搅拌72小时时Ag-NPs的稳定性增加。傅里叶变换红外（FT-IR）光谱表明GSH与Ag-NPs之间存在络合作用。使用肽等绿色化学试剂，为这项工作提供了绿色和经济的特性。
在不同搅拌时间的适中温度下，成功在GSH水溶液中合成了Ag-NPs。该研究清楚地表明，长时间搅拌合成的Ag-NPs，因此GSH反应的动力学非常缓慢。TEM结果表明，随着搅拌时间的增加，Ag-NPs的平均粒径增大。FT-IR光谱表明GSH与Ag-NPs之间存在络合作用。这些表明Ag-NPs可作为一种有效的细菌抑制剂，并可应用于医学领域。
