Department of Medical Applications of Laser, Pharmaceutical Technology Unit, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt.
Physics Department, Faculty of Science, Banha University, Banha, Egypt.
Int J Nanomedicine. 2020 Apr 17;15:2605-2615. doi: 10.2147/IJN.S250042. eCollection 2020.
This paper introduces a simple one-step and ultra-fast method for synthesis of highly photothermally active polypyrrole-coated gold nanoparticles. The synthesis process is so simple that the reaction is very fast without the need for any additives or complicated steps.
Polypyrrole-coated gold nanoparticles (AuPpy NPs) were synthesized by reacting chloroauric acid (HAuCl) with pyrrole (monomer) in aqueous medium at room temperature. These nanoparticles were characterized by UV-visible-NIR spectrometry, transmission electron microscopy (TEM), AC conductivity, zeta sizer and were evaluated for dark cytotoxicity and photocytotoxicity using human hepatocellular carcinoma (HepG2) cell line as a model for cancer cells.
The synthesized AuPpy NPs showed a peak characteristic for gold nanoparticles (530-600 nm, molar ratio dependent) and a wide absorption band along the visible-NIR region with intensity about triple or even quadruple that of polypyrrole synthesized by the conventional FeCl method at the same concentration and under the same conditions. TEM imaging showed that the synthesized AuPpy NPs were composed of spherical or semi-spherical gold core(s) of about 4-10 nm coated with distinct layer(s) of polypyrrole seen either loosely or in clusters. Mean sizes of the synthesized nanoparticles range between ~25 and 220 nm (molar ratio dependent). Zeta potentials of the AuPpy NPs preparations indicate their good colloidal stability. AC conductivity values of AuPpy NPs highly surpass that of Ppy prepared by the conventional FeCl method. AuPpy NPs were non-toxic even at high concentrations (up to 1000 µM pyrrole monomer equivalent) under dark conditions. Unlikely, light activated the photothermal activity of AuPpy NPs in a dose-dependent manner.
This method simply and successfully synthesized AuPpy NPs nanoparticles that represent a safe alternative photothermally active multifunctional tool instead of highly toxic and non-biodegradable gold nanorods.
本文介绍了一种简单的一步法和超快法合成具有高光热活性的聚吡咯包覆金纳米粒子。该合成过程非常简单，反应速度非常快，无需添加任何添加剂或复杂步骤。
在室温下，将氯金酸（HAuCl）与吡咯（单体）在水介质中反应，合成聚吡咯包覆的金纳米粒子（AuPpy NPs）。通过紫外-可见-近红外光谱、透射电子显微镜（TEM）、交流导率、Zeta 粒径仪对这些纳米粒子进行了表征，并用人肝癌（HepG2）细胞系作为癌细胞模型，评价了其暗毒性和光毒性。
合成的 AuPpy NPs 表现出金纳米粒子的特征峰（530-600nm，与摩尔比有关）和沿可见-近红外区域的宽吸收带，其强度是在相同浓度和相同条件下用传统的 FeCl 法合成的聚吡咯的三倍甚至四倍。TEM 成像显示，合成的 AuPpy NPs 由约 4-10nm 的球形或半球形金核组成，表面包覆有明显的聚吡咯层，或疏松或团聚。合成纳米粒子的平均粒径在25nm 到220nm 之间（与摩尔比有关）。AuPpy NPs 制剂的 Zeta 电位表明其胶体稳定性良好。AuPpy NPs 的交流导率值远高于传统 FeCl 法制备的 Ppy。在黑暗条件下，即使在高浓度（高达 1000µM 吡咯单体当量）下，AuPpy NPs 也没有毒性。相反，光以剂量依赖的方式激活了 AuPpy NPs 的光热活性。
该方法简单而成功地合成了 AuPpy NPs 纳米粒子，为安全替代高毒性和不可生物降解的金纳米棒提供了一种有前途的光热活性多功能工具。
