类风湿性关节炎核抗原抗体与对应于EB病毒核抗原1部分的合成肽的反应。
Reaction of antibodies to rheumatoid arthritis nuclear antigen with a synthetic peptide corresponding to part of Epstein-Barr nuclear antigen 1.
作者信息
Venables P J, Pawlowski T, Mumford P A, Brown C, Crawford D H, Maini R N
机构信息
Division of Clinical Immunology, Kennedy Institute of Rheumatology, London.
出版信息
Ann Rheum Dis. 1988 Apr;47(4):270-9. doi: 10.1136/ard.47.4.270.
Antibodies to rheumatoid arthritis nuclear antigen (RANA) are detected by immunodiffusion (ID) and immunofluorescence (IF), though reports of the identity of the antigen(s) have been conflicting. In this study it is shown conclusively that ID and IF anti-RANA react with epitopes on Epstein-Barr nuclear antigen 1 (EBNA-1) and that the major epitope detected by immunofluorescence is represented by a synthetic peptide, P62, corresponding to part of EBNA-1. In an enzyme linked immunosorbent assay (ELISA) anti-P62 antibodies in 35 rheumatoid arthritis sera were threefold higher than those of 35 age and sex matched controls, with the highest levels occurring in young patients with active joint disease.
通过免疫扩散（ID）和免疫荧光（IF）可检测到针对类风湿性关节炎核抗原（RANA）的抗体，不过关于该抗原身份的报告一直存在矛盾。在本研究中，确凿地表明ID和IF抗RANA与爱泼斯坦-巴尔核抗原1（EBNA-1）上的表位发生反应，并且免疫荧光检测到的主要表位由一种合成肽P62代表，该肽对应于EBNA-1的一部分。在酶联免疫吸附测定（ELISA）中，35例类风湿性关节炎血清中的抗P62抗体比35例年龄和性别匹配的对照者高两倍，最高水平出现在患有活动性关节疾病的年轻患者中。
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