Gorgievski-Hrisoho M, Hinderer W, Nebel-Schickel H, Horn J, Vornhagen R, Sonneborn H H, Wolf H, Siegl G
Division of Virology, Institute for Medical Microbiology, University of Bern, Switzerland.
J Clin Microbiol. 1990 Oct;28(10):2305-11. doi: 10.1128/jcm.28.10.2305-2311.1990.
Four recombinant, diagnostically useful Epstein-Barr virus (EBV) proteins representative of the viral capsid antigen (p150), diffuse early antigen (p54), the major DNA-binding protein (p138), and the EBV nuclear antigen (p72) (W. Hinderer, H. Nebel-Schickel, H.H. Sonneborn, M. Motz, R. Kühbeck, and H. Wolf, J. Exp. Clin. Cancer Res. 7[Suppl.]:132, 1988) were used to set up individual enzyme-linked immunosorbent assays (ELISAs) for the qualitative and quantitative detection of immunoglobulin M (IgM) and IgG antibodies. In direct comparison with results obtained by standard immunofluorescence or immunoperoxidase assays, it was then shown that the recombinant EBV ELISAs provide the means for specific and sensitive serodiagnosis of infectious mononucleosis (IM) caused by EBV. The most useful markers in sera from such patients proved to be IgM antibodies against p54, p138, and p150. Additional positive markers for recent or ongoing IM apparently were IgG antibodies against p54 and p138. In contrast, anti-p72 IgG had a high preference for sera from healthy blood donors and, therefore, can be considered indicative of past exposure to the virus. Altogether, the individual ELISAs proved to be as specific and at least as sensitive for the diagnosis of IM as the currently available standard techniques are. Moreover, our findings suggest that, by combining individual test antigens, a workable ELISA system consisting of three assays (IgM against p54, p138, and p150; IgG against p54 and p138; and IgG against p72) can be established for the standardized rapid diagnosis of acute EBV infections.
四种具有诊断价值的重组爱泼斯坦-巴尔病毒（EBV）蛋白，分别代表病毒衣壳抗原（p150）、弥漫性早期抗原（p54）、主要DNA结合蛋白（p138）和EBV核抗原（p72）（W. 欣德雷尔、H. 内贝尔-施克尔、H.H. 松内伯恩、M. 莫茨、R. 屈贝克和H. 沃尔夫，《实验与临床癌症研究》7[增刊]：132，1988）被用于建立单独的酶联免疫吸附测定（ELISA），以定性和定量检测免疫球蛋白M（IgM）和IgG抗体。与通过标准免疫荧光或免疫过氧化物酶测定获得的结果直接比较后发现，重组EBV ELISA为EBV引起的传染性单核细胞增多症（IM）提供了特异性和灵敏的血清学诊断方法。这类患者血清中最有用的标志物被证明是针对p54、p138和p150的IgM抗体。近期或正在发生的IM的其他阳性标志物显然是针对p54和p138的IgG抗体。相比之下，抗p72 IgG在健康献血者的血清中具有很高的偏好性，因此可以被认为是过去接触过该病毒的指标。总体而言，单独的ELISA被证明与目前可用的标准技术一样具有特异性，并且在诊断IM方面至少同样灵敏。此外，我们的研究结果表明，通过组合单个检测抗原，可以建立一个由三种测定组成的可行的ELISA系统（针对p54、p138和p150的IgM；针对p54和p138的IgG；以及针对p72的IgG），用于急性EBV感染的标准化快速诊断。
