Witebsky F G, Andrews J W, Gill V J, MacLowry J D
National Institutes of Health, Bethesda, Maryland 20892.
J Clin Microbiol. 1988 Apr;26(4):774-5. doi: 10.1128/jcm.26.4.774-775.1988.
A modified toluidine blue O (TBO) stain for Pneumocystis carinii cysts was evaluated with regard to the influence of (i) the age and extent of use of the sulfation reagent, (ii) the source of TBO, (iii) the TBO content of the staining solution, and (iv) the amount of TBO present in the alcohol wash solutions. All TBOs evaluated, except for a new TBO obtained from Roboz Surgical Instrument Co., Inc., Washington, D.C., produced satisfactory results. Each lot of TBO should be quality controlled before use to ensure that the P. carinii cysts stain lavender against a blue background. We have ourselves decided to use only certified TBO with a high dye content. As extensively used sulfation reagent provided less satisfactory results than did either freshly prepared or 1-week-old unused sulfation reagent, we have decided to prepare fresh sulfation reagent at least weekly and to discard used sulfation reagent after 10 slides have been processed.
对一种改良的甲苯胺蓝O（TBO）染色法进行了评估，该方法用于卡氏肺孢子虫包囊染色，评估内容包括：（i）硫酸化试剂的使用年限和使用程度；（ii）TBO的来源；（iii）染色液中的TBO含量；（iv）酒精冲洗液中的TBO含量。除了从华盛顿特区的Roboz Surgical Instrument Co., Inc.获得的一种新型TBO外，所有评估的TBO均产生了满意的结果。每批TBO在使用前都应进行质量控制，以确保卡氏肺孢子虫包囊在蓝色背景下染成淡紫色。我们自己决定只使用染料含量高的认证TBO。由于广泛使用的硫酸化试剂产生的结果不如新鲜制备的或未使用1周的硫酸化试剂，我们决定至少每周制备新鲜的硫酸化试剂，并在处理10张玻片后丢弃用过的硫酸化试剂。
