CSIR - Central Electrochemical Research Institute, Karaikudi 630006, Tamil Nadu, India.
CSIR - Central Electrochemical Research Institute, Karaikudi 630006, Tamil Nadu, India.
Carbohydr Polym. 2014 Mar 15;103:448-55. doi: 10.1016/j.carbpol.2013.12.074. Epub 2014 Jan 5.
ZnO nanoparticles (NPs) synthesis on cotton fabric through the formation of biologically activated ammonia from urea broth in the presence of the ureolytic bacterial species Serratia ureilytica (HM475278) has been described in the present contribution. The cotton fabric was immersed in biogenic zinc ammonium complex medium and subjected to heat treatment at an optimum temperature of 50 °C for different durations of time (30, 60, 90 min). The crystal growth of ZnO nanoparticles on cotton fabric was characterized by analytical techniques such as SEM-EDAX, XRD, TGA, CHNS and UV-visible spectra, and evaluation of antibacterial activity was carried out against Escherichia coli and Staphylococcus aureus. Crystal growth and morphological studies confirmed the attachment of ZnO NPs on the cotton fabric. Spherical to nanoflower shaped particles were obtained with increasing time duration from 30 to 90 min. The antibacterial activity of loaded cotton fabrics was found to be substantially higher than the bare cotton samples. Wet film interfacial contact studies have shown greater antibacterial activity as a result of nanoparticle contact at the bio-interface, as observed by Epi-fluorescent microscopic observations.
本研究描述了通过在脲酶细菌物种变形菌（HM475278）存在下从尿素肉汤中形成生物激活的氨，在棉织物上合成氧化锌纳米颗粒（NPs）。将棉织物浸入生物成因的锌氨络合物介质中，并在最佳温度 50°C 下进行热处理不同的时间（30、60、90 分钟）。通过 SEM-EDAX、XRD、TGA、CHNS 和 UV-可见光谱等分析技术对棉织物上氧化锌纳米颗粒的晶体生长进行了表征，并对大肠杆菌和金黄色葡萄球菌进行了抗菌活性评价。晶体生长和形态研究证实了 ZnO NPs 附着在棉织物上。随着时间从 30 分钟增加到 90 分钟，得到了球形到纳米花状的颗粒。负载棉织物的抗菌活性明显高于裸棉样品。湿膜界面接触研究表明，由于在生物界面处的纳米颗粒接触，抗菌活性更高，这可以通过荧光显微镜观察到。
