Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Curr Opin Chem Biol. 2014 Feb;18:21-8. doi: 10.1016/j.cbpa.2013.09.020. Epub 2013 Nov 27.
High-throughput and unbiased binding assays have proven useful in probe discovery for a myriad of biomolecules, including targets of unknown structure or function and historically challenging target classes. Over the past decade, a number of novel formats for executing large-scale binding assays have been developed and used successfully in probe discovery campaigns. Here we review the use of one such format, the small-molecule microarray (SMM), as a tool for discovering protein-small molecule interactions. This review will briefly highlight selected recent probe discoveries using SMMs as well as novel uses of SMMs in profiling applications.
高通量且无偏倚的结合测定法已被证明在探测各种生物分子，包括未知结构或功能的靶标和历史上具有挑战性的靶标类别方面非常有用。在过去的十年中，已经开发并成功地在探测发现计划中使用了许多用于执行大规模结合测定法的新型格式。在这里，我们回顾了一种这样的格式，即小分子微阵列（SMM），作为发现蛋白质-小分子相互作用的工具。这篇综述将简要地强调使用 SMM 进行的一些最新探针发现，以及 SMM 在分析应用中的新用途。
