Unit of Viral Diseases and Attenuated Vaccines, and Unit of Vector-Borne Diseases and International Health, Department of Infectious, Parasitic and Immunomediated Disease, and Global Health Unit, Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Rome, Italy.
Am J Trop Med Hyg. 2014 Apr;90(4):760-3. doi: 10.4269/ajtmh.13-0457. Epub 2014 Feb 17.
Thirty pools from 900 (540 females and 360 males) Phlebotomus perfiliewi sandflies collected during the summer of 2012 in the Fermo area (Marche Region, central Italy) were tested for the presence of Phleboviruses. A nested polymerase chain reaction was performed using degenerated primers amplifying a fragment of the polymerase gene (large segment) and a fragment of the nucleoprotein gene (small segment) of the genus Phlebovirus. One pool was positive for Toscana virus, as expected from results of studies in the area, and six pools were positive for a putative novel Phlebovirus. Virus isolation in Vero cells was performed. Minimum field infection rates/1,000 insects processed for the novel and Toscana viruses were 6.7 and 1.0, respectively. Phylogenetic analysis of the novel Phlebovirus, tentatively named Fermo virus, placed it in the Sandfly Fever Naples virus serocomplex.
2012 年夏季，在意大利中部马尔凯地区费尔莫地区采集了 900 只（540 只雌性和 360 只雄性）白蛉属（Phlebotomus perfiliewi）沙蝇，对其进行了检测以确定是否存在黄病毒属（Phlebovirus）。使用简并引物进行巢式聚合酶链反应，扩增聚合酶基因（大片段）和核蛋白基因（小片段）的片段。一个沙蝇种群检测出了托斯卡纳病毒，这与该地区的研究结果一致，另外 6 个沙蝇种群检测出了一种可能的新型黄病毒。在vero 细胞中进行病毒分离。新型和托斯卡纳病毒的最低野外感染率/处理的 1000 只昆虫分别为 6.7 和 1.0。新型黄病毒（暂命名为费尔莫病毒）的系统发育分析将其置于沙蝇热那不勒斯病毒血清群中。
