Ciufolini M G, Maroli M, Guandalini E, Marchi A, Verani P
Istituto Superiore di Sanità, Rome, Italy.
Am J Trop Med Hyg. 1989 Jun;40(6):669-75. doi: 10.4269/ajtmh.1989.40.669.
Transovarial transmission (TOT) of Toscana (TOS) and Arbia (ARB) viruses in a laboratory colony of Phlebotomus perniciosus is reported. Toscana and ARB viruses were maintained in P. perniciosus females, initially infected by intrathoracic inoculation, for 2 and 3 consecutive generations respectively. TOT was demonstrated in F1 (75%) and F2 (67%) generation adults for TOS and F1 (47%), F2 (37%), and F3 (34%) generation adults for ARB virus. The progressive decline of virus infection rates in each generation suggests that these agents cannot be maintained indefinitely by TOT. No infection was observed in F1 progeny after female parents were fed through membranes with either virus. Transovarially infected females were able to transmit TOS virus by bite to a susceptible vertebrate. Venereal infection of P. perniciosus females mated to males transovarially infected with TOS virus was seen.
据报道，在实验室饲养的白蛉种群中，托斯卡纳（TOS）病毒和阿尔比亚（ARB）病毒可经卵传播（TOT）。托斯卡纳病毒和ARB病毒分别在最初通过胸腔接种感染的雌性白蛉体内连续传代2代和3代。TOS病毒在F1代（75%）和F2代（67%）成虫中证实存在经卵传播，ARB病毒在F1代（47%）、F2代（37%）和F3代（34%）成虫中证实存在经卵传播。每一代病毒感染率的逐渐下降表明，这些病原体不能通过经卵传播无限期维持。在用任何一种病毒通过膜饲喂雌性亲代后，在F1代子代中均未观察到感染。经卵感染的雌性白蛉能够通过叮咬将TOS病毒传播给易感脊椎动物。观察到与经卵感染TOS病毒的雄性白蛉交配的雌性白蛉发生了性感染。
