Jafarzadeh Nazli, Javeri Arash, Khaleghi Maryam, Taha Masoumeh Fakhr
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd., P.O. Box: 14965-161, Tehran, Iran.
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd., P.O. Box: 14965-161, Tehran, Iran.
Neurosci Lett. 2014 Apr 3;564:105-10. doi: 10.1016/j.neulet.2014.02.012. Epub 2014 Feb 16.
The neurohypophyseal hormone oxytocin plays a role in stimulation of neurogenesis in the adult brain. However, the exact role of oxytocin in neural development is still not well understood. In the present study, we evaluated the effect of oxytocin on neural differentiation of mouse adipose tissue-derived stem cells (ADSCs). For this purpose, ADSCs were cultured in a medium containing Knockout™ Serum Replacement (KoSR) and treated with different concentrations of oxytocin at the first or eighth day of differentiation. Two weeks after neural induction, ADSCs expressed several early and late neuron-specific genes and proteins. MTT assay and cell cycle analysis revealed a stimulatory effect of oxytocin on viability and proliferation of differentiating ADSCs. As detected by quantitative real-time PCR, treatment of the ADSCs with low concentrations of oxytocin induced neurogenesis. Oxytocin treatment also upregulated the expression of oxytocin receptor mRNA. These results demonstrated for the first time that oxytocin treatment can promote neural differentiation of the ADSCs in a dose-dependent and time-dependent manner. Oxytocin has a significant role in neurogenesis, and this may have implications in regeneration of adult neurons.
神经垂体激素催产素在成体大脑神经发生的刺激过程中发挥作用。然而，催产素在神经发育中的确切作用仍未得到充分理解。在本研究中，我们评估了催产素对小鼠脂肪组织来源干细胞（ADSCs）神经分化的影响。为此，将ADSCs培养于含有Knockout™血清替代物（KoSR）的培养基中，并在分化的第一天或第八天用不同浓度的催产素进行处理。神经诱导两周后，ADSCs表达了几种早期和晚期神经元特异性基因及蛋白质。MTT分析和细胞周期分析显示催产素对分化中的ADSCs的活力和增殖具有刺激作用。通过定量实时PCR检测发现，用低浓度催产素处理ADSCs可诱导神经发生。催产素处理还上调了催产素受体mRNA的表达。这些结果首次证明，催产素处理可通过剂量依赖性和时间依赖性方式促进ADSCs的神经分化。催产素在神经发生中具有重要作用，这可能对成体神经元的再生具有意义。
