Brango-Vanegas J, Costa G M, Ortmann C F, Schenkel E P, Reginatto F H, Ramos F A, Arévalo-Ferro C, Castellanos L
Departamento de Química, Universidad Nacional de Colombia, Ciudad Universitaria, edificio 451, Carrera 30 N° 45-03, Bogotá, Colombia.
Departamento de Química, Universidad Nacional de Colombia, Ciudad Universitaria, edificio 451, Carrera 30 N° 45-03, Bogotá, Colombia; Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, CEP 88040-970, Florianópolis, SC, Brazil.
Phytomedicine. 2014 Apr 15;21(5):670-5. doi: 10.1016/j.phymed.2014.01.001. Epub 2014 Feb 16.
The Cecropia genus is widely distributed in Latin America including at least 60 species, and some of them are commonly used in traditional medicine for the treatment of several diseases. We used Cecropia pachystachya Trécul to search for quorum sensing (QS) inhibitors compounds and found that the aqueous extract of C. pachystachya leaves is a promising source of substances with this activity. Using as biosensor Chromobacterium violaceum ATCC 31532 and Escherichia coli pSB403, the compounds chlorogenic acid (2), isoorientin (3), orientin (4), isovitexin (6), vitexin (7), and rutin (9) were identified as QS inhibitors. None of these compounds inhibited the growth of neither the used biosensors nor the microorganisms Staphylococcus aureus ATCC 23591, Escherichia coli ATCC 25922 and Saccharomyces cerevisiae, used here as growth inhibition controls. Along with the rutin, here we presented for the first time the QS-inhibition potential of the C-glycosyl flavonoids. The prospective of this evidence lead to the use of these compounds as antipathogenic drugs or antifoulants.
蚁栖树属广泛分布于拉丁美洲，包括至少60个物种，其中一些在传统医学中常用于治疗多种疾病。我们利用墨西哥蚁栖树来寻找群体感应（QS）抑制剂化合物，发现墨西哥蚁栖树叶子的水提取物是具有这种活性物质的一个有前景的来源。使用紫色杆菌ATCC 31532和大肠杆菌pSB403作为生物传感器，鉴定出绿原酸（2）、异荭草素（3）、荭草素（4）、异牡荆素（6）、牡荆素（7）和芦丁（9）为QS抑制剂。这些化合物均未抑制所用生物传感器的生长，也未抑制金黄色葡萄球菌ATCC 23591、大肠杆菌ATCC 25922和酿酒酵母的生长，此处将这些微生物用作生长抑制对照。除芦丁外，我们在此首次展示了C-糖基黄酮类化合物的QS抑制潜力。这一证据的前景促使这些化合物用作抗病原药物或防污剂。
