Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria; Omics Center Graz, Austria.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 1;951-952:119-28. doi: 10.1016/j.jchromb.2014.01.011. Epub 2014 Jan 29.
A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.
介绍了一种基于 UPLC 的新型非靶向脂质组学方法，使用 qTOF 混合质谱仪。应用的二元梯度可实现包括构象异构体化合物和低丰度脂质类（如磷脂酸（PA））在内的脂质种类的分离。在溶剂中添加磷酸可改善酸性磷脂的峰形。MS(E)扫描允许同时获取全扫描数据和碰撞诱导碎裂，以提高脂质种类的鉴定能力并获得结构信息。该方法用于研究酵母的脂质组。
