Merkhofer Evan C, Hu Peter, Johnson Tracy L
Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA.
Methods Mol Biol. 2014;1126:83-96. doi: 10.1007/978-1-62703-980-2_6.
The discovery that many intron-containing genes can be cotranscriptionally spliced has led to an increased understanding of how splicing and transcription are intricately intertwined. Cotranscriptional splicing has been demonstrated in a number of different organisms and has been shown to play roles in coordinating both constitutive and alternative splicing. The nature of cotranscriptional splicing suggests that changes in transcription can dramatically affect splicing, and new evidence suggests that splicing can, in turn, influence transcription. In this chapter, we discuss the mechanisms and consequences of cotranscriptional splicing and introduce some of the tools used to measure this process.
许多含内含子的基因能够进行共转录剪接这一发现，增进了我们对剪接与转录如何复杂交织的理解。共转录剪接已在多种不同生物中得到证实，并显示在协调组成型剪接和可变剪接中发挥作用。共转录剪接的性质表明，转录变化可显著影响剪接，而新证据表明，剪接反过来也可影响转录。在本章中，我们将讨论共转录剪接的机制和后果，并介绍一些用于测量这一过程的工具。
