Avula Pulla Reddy, Asdaq Syed Mohammed, Asad Mohammed
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore, Karnataka, India.
Department of Pharmacology, Al- Maarefa College for Science and Technology, Riyadh, Saudi Arabia.
Indian J Pharmacol. 2014 Jan-Feb;46(1):94-9. doi: 10.4103/0253-7613.125185.
The study evaluates the cardioprotective effect of aged garlic extract (AGE) and its constituent; S-allylcysteine (SAC) and their interaction with atenolol during isoproterenol induced cardiac toxicity in rats.
Rats were administered AGE at two different doses of 2 ml/kg or 5 ml/kg orally whereas SAC was administered either at a dose 13.1 mg/kg or 32.76 mg/kg. The AGE or SAC was given alone or in combination with atenolol (6 mg/kg, p.o), every alternate day for three weeks. At the end of treatment, two doses of isoproterenol (150 mg/kg, s.c) were administered to rats. The electrocardiogram (ECG) was recorded followed by withdrawal of blood to estimate serum lactate dehydrogenase (LDH) and creatinine kinase-MB (CK-MB) activities. The activities of LDH, CK-MB as well as superoxide dismutase (SOD), catalase and thiobarbituric acid reactive substances (TBARS) were also determined in the heart tissue homogenate (HTH).
The isoproterenol induced ECG changes were restored to normal in all treated groups. The AGE and SAC administration caused a decrease in serum LDH and CK-MB activities and an elevation of LDH and CK-MB activities in HTH. Atenolol alone or in combination with AGE and S-allylcsyteine demonstrated similar changes in biomarker activities.
AGE showed dose-dependent cardioprotection. However, concurrent administration of SAC with atenolol (6 mg/kg, p.o) combated more effectively the myocardial dysfunction during isoproterenol induced cardiotoxicity in rats.
本研究评估了 aged garlic extract（AGE）及其成分 S-烯丙基半胱氨酸（SAC）在异丙肾上腺素诱导的大鼠心脏毒性过程中的心脏保护作用，以及它们与阿替洛尔的相互作用。
大鼠分别口服两种不同剂量（2 ml/kg 或 5 ml/kg）的 AGE，而 SAC 的给药剂量为 13.1 mg/kg 或 32.76 mg/kg。AGE 或 SAC 单独给药或与阿替洛尔（6 mg/kg，口服）联合给药，每隔一天给药一次，持续三周。治疗结束时，给大鼠注射两剂异丙肾上腺素（150 mg/kg，皮下注射）。记录心电图（ECG），随后取血以估计血清乳酸脱氢酶（LDH）和肌酸激酶-MB（CK-MB）活性。还测定了心脏组织匀浆（HTH）中 LDH、CK-MB 以及超氧化物歧化酶（SOD）、过氧化氢酶和硫代巴比妥酸反应性物质（TBARS）的活性。
所有治疗组中，异丙肾上腺素诱导的 ECG 变化均恢复正常。给予 AGE 和 SAC 导致血清 LDH 和 CK-MB 活性降低，而 HTH 中 LDH 和 CK-MB 活性升高。单独使用阿替洛尔或与 AGE 和 S-烯丙基半胱氨酸联合使用时，生物标志物活性表现出类似变化。
AGE 显示出剂量依赖性的心脏保护作用。然而，在异丙肾上腺素诱导的大鼠心脏毒性过程中，SAC 与阿替洛尔（6 mg/kg，口服）联合给药能更有效地对抗心肌功能障碍。
