Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah, Riyadh 13713, Saudi Arabia.
Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore 560035, India.
Molecules. 2021 May 27;26(11):3203. doi: 10.3390/molecules26113203.
This study was conducted to determine the potential interaction of aged garlic extract (AGE) with carvedilol (CAR), as well as to investigate the role of S-allyl-l-cysteine (SAC), an active constituent of AGE, in rats with isoproterenol (ISO)-induced myocardial dysfunction. At the end of three weeks of treatment with AGE (2 and 5 mL/kg) or SAC (13.1 and 32.76 mg/kg), either alone or along with CAR (10 mg/kg) in the respective groups of animals, ISO was administered subcutaneously to induce myocardial damage. Myocardial infarction (MI) diagnostic predictor enzymes, lactate dehydrogenase (LDH) and creatinine kinase (CK-MB), were measured in both serum and heart tissue homogenates (HTH). Superoxide dismutase (SOD), catalase, and thiobarbituric acid reactive species (TBARS) were estimated in HTH. When compared with other groups, the combined therapy of high doses of AGE and SAC given alone or together with CAR caused a significant decrease in serum LDH and CK-MB activities. Further, significant rise in the LDH and CK-MB activities in HTH was noticed in the combined groups of AGE and SAC with CAR. It was also observed that both doses of AGE and SAC significantly increased endogenous antioxidants in HTH. Furthermore, histopathological observations corroborated the biochemical findings. The cytoprotective potential of SAC and AGE were dose-dependent, and SAC was more potent than AGE. The protection offered by aged garlic may be attributed to SAC. Overall, the results indicated that a high dose of AGE and its constituent SAC, when combined with carvedilol, has a synergistic effect in preventing morphological and physiological changes in the myocardium during ISO-induced myocardial damage.
这项研究旨在确定陈年大蒜提取物（AGE）与卡维地洛（CAR）的潜在相互作用，并研究AGE 的活性成分 S-烯丙基-L-半胱氨酸（SAC）在异丙肾上腺素（ISO）诱导的心肌功能障碍大鼠中的作用。在三周的 AGE（2 和 5 mL/kg）或 SAC（13.1 和 32.76 mg/kg）治疗（单独或与 CAR 一起在动物的相应组中）结束后，皮下给予 ISO 以诱导心肌损伤。测量血清和心肌组织匀浆（HTH）中的心肌梗死（MI）诊断预测酶，乳酸脱氢酶（LDH）和肌酸激酶同工酶（CK-MB）。在 HTH 中估计超氧化物歧化酶（SOD）、过氧化氢酶和硫代巴比妥酸反应物质（TBARS）。与其他组相比，单独或与 CAR 一起给予高剂量的 AGE 和 SAC 的联合治疗导致血清 LDH 和 CK-MB 活性显着降低。此外，在 AGE 和 SAC 与 CAR 的联合组中，还观察到 HTH 中 LDH 和 CK-MB 活性显着升高。还观察到，两种剂量的 AGE 和 SAC 显着增加了 HTH 中的内源性抗氧化剂。此外，组织病理学观察证实了生化发现。SAC 和 AGE 的细胞保护潜力呈剂量依赖性，SAC 比 AGE 更有效。陈年大蒜提供的保护作用可能归因于 SAC。总的来说，结果表明，高剂量的 AGE 及其成分 SAC 与卡维地洛联合使用，在 ISO 诱导的心肌损伤期间具有协同作用，可以预防心肌形态和生理变化。
