Bezbaruah R L, Pillai K R, Gogoi B K, Baruah J N
Biochemistry Division, Regional Research Laboratory, Assam, India.
Antonie Van Leeuwenhoek. 1988;54(1):37-45. doi: 10.1007/BF00393956.
The influence of growth temperature, media composition and cell age on the chemical composition of Bacillus stearothermophilus strain AN 002 has been determined. The total cellular protein decreased and the free amino acid content increased with growth temperature, in both exponential and stationary growth phase. The protein and free amino acid contents of cells were higher in the stationary phase than in the exponential phase, irrespective of growth temperature and media composition. The RNA content was only reduced in cells grown at 55 degrees C. No significant variations were observed in the DNA and carbohydrate contents with respect to growth temperature and cell age. The total lipid and fatty acid compositions on the other hand varied as a function of growth temperature, cell age and media composition. Differences in the relative concentrations of even, odd and branched chain fatty acids were noticed. No variation was observed in the antiiso and unsaturated fatty acids with respect to growth temperature. The unique variations in the fatty acid composition and total lipids at the growth temperature of 50 degrees C and their variations in the stationary growth phase seem to be characteristic for B. stearothermophilus AN 002.
已测定生长温度、培养基成分和细胞年龄对嗜热脂肪芽孢杆菌菌株AN 002化学成分的影响。在指数生长期和稳定生长期，随着生长温度升高，细胞总蛋白含量下降，游离氨基酸含量增加。无论生长温度和培养基成分如何，稳定期细胞的蛋白质和游离氨基酸含量均高于指数生长期。仅在55℃生长的细胞中RNA含量降低。关于生长温度和细胞年龄，未观察到DNA和碳水化合物含量有显著变化。另一方面，总脂质和脂肪酸组成随生长温度、细胞年龄和培养基成分而变化。注意到偶数、奇数和支链脂肪酸相对浓度存在差异。关于生长温度，反异脂肪酸和不饱和脂肪酸未观察到变化。在50℃生长温度下脂肪酸组成和总脂质的独特变化及其在稳定生长期的变化似乎是嗜热脂肪芽孢杆菌AN 002的特征。
