Aerts J M, Lauwers A M, Heinen W
Antonie Van Leeuwenhoek. 1985;51(2):155-65. doi: 10.1007/BF02310009.
The lipid content and fatty acid composition of a strain of Bacillus caldolyticus and of two facultative thermophiles (B. flavothermus and strain NZ-2) were analysed after growth at different temperatures. In all three strains the amount of membrane, as a fraction of total cellular dry mass, was found to increase with temperature, however, in varying degrees. Changes of lipid content and protein/lipid ratio in B. caldolyticus between 60 degrees C and 100 degrees C and in strain NZ-2 between 45 degrees C and 70 degrees C were minor; in B. flavothermus the alterations in the 50 degrees C-70 degrees C range were more pronounced. The same was found for changes observed in the phospholipid/total lipid and phospholipid/membrane ratios, and also in the amounts of individual phospholipids. The alterations of the fatty acid composition were most significant in B. caldolyticus, especially between 80 degrees C and 95 degrees C. In contrast, the main changes in B. flavothermus and NZ-2 were found to occur between 30 degrees C and 50 degrees C, and between 45 degrees C and 60 degrees C, respectively. Based on these data, strain NZ-2 could be characterized as the least and B. flavothermus as the most versatile of the three organisms.
对嗜热解硫芽孢杆菌菌株以及两种兼性嗜热菌（嗜热栖热菌和NZ-2菌株）在不同温度下生长后的脂质含量和脂肪酸组成进行了分析。在所有这三种菌株中，作为细胞总干重一部分的膜含量被发现会随着温度升高而增加，不过增加程度各不相同。嗜热解硫芽孢杆菌在60℃至100℃之间以及NZ-2菌株在45℃至70℃之间，脂质含量和蛋白质/脂质比的变化较小；嗜热栖热菌在50℃至70℃范围内的变化更为明显。在磷脂/总脂质和磷脂/膜比率以及各个磷脂的含量方面观察到的变化也是如此。脂肪酸组成的变化在嗜热解硫芽孢杆菌中最为显著，尤其是在80℃至95℃之间。相比之下，嗜热栖热菌和NZ-2的主要变化分别发生在30℃至50℃之间以及45℃至60℃之间。基于这些数据，NZ-2菌株可被表征为这三种生物中适应性最差的，而嗜热栖热菌则是适应性最强的。
