Tofighi Zahra, Alipour Fatemeh, Hadavinia Hormoz, Abdollahi Mohammad, Hadjiakhoondi Abbas, Yassa Narguess
Department of Pharmacognosy, Faculty of Pharmacy, Medicinal Plant Research Center, Tehran University of Medical Sciences , Tehran , Iran and.
Pharm Biol. 2014 Aug;52(8):961-6. doi: 10.3109/13880209.2013.874463. Epub 2014 Feb 20.
Otostegia persica (Burm.) Boiss. (Lamiaceae), "Goldar" in Persian, is widely used in the folk medicine of south Iran for control of diabetes mellitus.
In the present study, hypoglycemic and antioxidant effects of different fractions of the O. persica extract were investigated and constituents of effective fractions were elucidated.
Different concentrations (100-400 mg/kg) of aqueous infusion (AI) of flowering aerial parts of the plant (traditional preparation) and all fractions of the O. persica extract (i.p. injection) were tested for antidiabetic activity in streptozocin-induced diabetic NMRI mice. Blood glucose level was measured at time 0 and intervals of 1, 2, 4, and 6 h later. Antioxidant activities of different fractions of the plant extract and pure compounds (0.1, 0.5, and 1 mg/ml) were determined with the DPPH method. Four compounds were isolated and identified from potent fractions.
Antidiabetic activity demonstrated that the effect of the methanol fraction at a dose of 300 mg/kg was equivalent with glibenclamide, and at a dose of 400 mg/kg was comparable with glibenclamide and insulin (p > 0.05). The EC50 of the methanol fraction was 307.12 mg. Methanol and ethyl acetate fractions showed antioxidant activities (both IC50 equal to 0.49 mg/ml), so these fractions were selected for the purification of compounds. Chrysoeriol from ethyl acetate and three apigenin derivatives (6-methylapigenin, apigenin-7-O-glucoside, and echinaticin) from the methanol fraction were isolated and identified (new for the species). Chrysoeriol exhibited potent antioxidant activity comparable with vitamin E and BHT (p > 0.05).
The present study confirmed the folklore usage of O. persica for antidiabetic properties.
波斯耳草（Otostegia persica (Burm.) Boiss.）（唇形科），波斯语称“Goldar”，在伊朗南部民间医学中被广泛用于控制糖尿病。
在本研究中，研究了波斯耳草提取物不同馏分的降血糖和抗氧化作用，并阐明了有效馏分的成分。
测试了植物开花地上部分的水浸液（AI）（传统制剂）的不同浓度（100 - 400 mg/kg）以及波斯耳草提取物的所有馏分（腹腔注射）对链脲佐菌素诱导的糖尿病NMRI小鼠的抗糖尿病活性。在0小时以及之后1、2、4和6小时的间隔时间测量血糖水平。用DPPH法测定植物提取物不同馏分和纯化合物（0.1、0.5和1 mg/ml）的抗氧化活性。从有效馏分中分离并鉴定出四种化合物。
抗糖尿病活性表明，甲醇馏分在300 mg/kg剂量下的效果与格列本脲相当，在400 mg/kg剂量下与格列本脲和胰岛素相当（p > 0.05）。甲醇馏分的半数有效浓度（EC50）为307.12 mg。甲醇和乙酸乙酯馏分显示出抗氧化活性（两者半数抑制浓度（IC50）均等于0.49 mg/ml），因此选择这些馏分进行化合物纯化。从乙酸乙酯中分离并鉴定出 Chrysoeriol，从甲醇馏分中分离并鉴定出三种芹菜素衍生物（6 - 甲基芹菜素、芹菜素 - 7 - O - 葡萄糖苷和紫锥菊素）（该物种新发现）。Chrysoeriol表现出与维生素E和丁基羟基甲苯相当的强抗氧化活性（p > 0.05）。
本研究证实了波斯耳草在民间用于抗糖尿病特性的用法。
