Sabernavaei Mahsa, Kobarfard Farzad, Hadjiakhoondi Abbas, Aghaahmadi Majid, Amin Mohsen, Yassa Narguess
Department of Pharmacognosy, Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Depatrtment of medicinal chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2018 Fall;17(4):1386-1391.
Mozaff. That belongs to Apiaceae family is an endemic species distributed in the west of Iran. The aim of this study was to investigate the antioxidant activity and acetylcholinestrase (AChE) inhibition of the crude extract, fractions, and isolated compounds from methanol fraction of Five compounds were detected from methanol fraction; three phenolic compounds as p-coumric acid, caffeic acid, salicylic acid and also, two flavonoids as quercetin and astragalin. These structures were identified by spectroscopic techniques such as H-NMR, C-NMR, and UV. Antioxidant activity was evaluated by the free radical scavenging assay using 2, 2-diphenyl-1-picryl- hydrazyl (DPPH) method. Ellman colorimetric method was used to determine acetylcholinestrase (AChE) inhibition. In the DPPH assay, Quercetin exerted the highest antioxidant activity (IC = 10.24 ± 1.3 µg/mL). Caffeic acid inhibited AChE with IC = 12.06 ± 2.01µg/mL which were comparable to Galanthamine as positive control (IC = 62.44 ± 2.2µg/mL). In conclusion, methanol extract of contains bioactive components with antioxidant and AChE inhibitory effects.
莫扎夫。它属于伞形科，是一种分布于伊朗西部的特有物种。本研究的目的是研究从[植物名称]甲醇提取物中得到的粗提物、馏分和分离化合物的抗氧化活性及对乙酰胆碱酯酶（AChE）的抑制作用。从甲醇馏分中检测到五种化合物；三种酚类化合物，即对香豆酸、咖啡酸、水杨酸，还有两种黄酮类化合物，即槲皮素和紫云英苷。这些结构通过诸如氢核磁共振（H-NMR）、碳核磁共振（C-NMR）和紫外（UV）等光谱技术进行鉴定。抗氧化活性通过使用2,2-二苯基-1-苦基肼（DPPH）法的自由基清除试验进行评估。采用埃尔曼比色法测定对乙酰胆碱酯酶（AChE）的抑制作用。在DPPH试验中，槲皮素表现出最高的抗氧化活性（IC₅₀ = 10.24 ± 1.3微克/毫升）。咖啡酸对AChE的抑制作用IC₅₀ = 12.06 ± 2.01微克/毫升，与作为阳性对照的加兰他敏（IC₅₀ = 62.44 ± 2.2微克/毫升）相当。总之，[植物名称]的甲醇提取物含有具有抗氧化和AChE抑制作用的生物活性成分。
