Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Biomed Res Int. 2017;2017:7319504. doi: 10.1155/2017/7319504. Epub 2017 Sep 10.
In an attempt to identify herbal drugs which may become useful in the prevention of diabetes, antioxidant potentials and -amylase inhibition by the ethanol extracts of two plants belonging to Lamiaceae family, and , and their different fractions were studied. Also, inhibition of -amylase by and its fractions was evaluated. All of the samples exhibited antioxidant activities, among which ethyl acetate fraction of (17.21 ± 0.17 mg GAE/g) was found to contain the highest amounts of phenols and the ethyl acetate fraction of (218 ± 2.76 mg QUE/g) had the most values of flavonoids. Ethyl acetate fraction of (IC = 3.05 ± 0.51 g/ml) was shown to have the most reducing power and the ethyl acetate fraction of (IC = 32.17 ± 1.82 g/ml) exhibited the highest DPPH radical scavenging. The ethyl acetate fraction of (99.39 ± 0.94%) showed the highest -amylase inhibitory activity which was similar to acarbose used as a standard. Mode of -amylase inhibition of the most samples was uncompetitive except for , , , and which presented competitive inhibition. The present findings showed that studied samples may have some compounds with antioxidant and antidiabetic effects.
为了寻找可能有助于预防糖尿病的草药，研究了唇形科的两种植物， 和 ，及其不同部位的乙醇提取物的抗氧化潜力和 -淀粉酶抑制作用。还评估了 和其馏分对 -淀粉酶的抑制作用。所有样品均表现出抗氧化活性，其中 （17.21 ± 0.17 mg GAE/g）的乙酸乙酯部分酚类含量最高， （218 ± 2.76 mg QUE/g）的乙酸乙酯部分黄酮类含量最高。 （IC = 3.05 ± 0.51 g/ml）的乙酸乙酯部分具有最强的还原能力， （IC = 32.17 ± 1.82 g/ml）的乙酸乙酯部分对 DPPH 自由基的清除能力最强。 （99.39 ± 0.94%）的乙酸乙酯部分对 -淀粉酶的抑制活性最高，与作为标准的阿卡波糖相似。除了 、 、 和 之外，大多数样品的 -淀粉酶抑制模式为非竞争性，而这四种样品则呈现竞争性抑制。本研究结果表明，研究的样品可能含有一些具有抗氧化和抗糖尿病作用的化合物。
