盘尾丝虫重组抗原：物理特性及其与血清反应性的临床相关性

Onchocerca volvulus recombinant antigen: physical characterization and clinical correlates with serum reactivity.

作者信息

Gallin M Y, Tan M, Kron M A, Rechnitzer D, Greene B M, Newland H S, White A T, Taylor H R, Unnasch T R

机构信息

Department of Medicine, Case Western Reserve University, Cleveland, Ohio.

出版信息

J Infect Dis. 1989 Sep;160(3):521-9. doi: 10.1093/infdis/160.3.521.

DOI:10.1093/infdis/160.3.521

PMID:2760503

Abstract

An Onchocerca volvulus cDNA clone expressing epitopes found in adult and larval parasites, designated lambda RAL-2, was derived from a 1,000-base message present in adult O. volvulus, which encodes a protein with an apparent molecular weight of 17,000. This protein does not appear to be extensively posttranslationally modified. Serum samples from 52 individuals exposed to O. volvulus were examined for antibodies recognizing the lambda RAL-2 recombinant antigen; 77% produced such antibodies. In addition, individuals producing antibodies recognizing the recombinant antigen were significantly less likely to develop some aspects of ocular pathology associated with O. volvulus infection than were individuals who did not do so. These results suggest that recombinant antigens such as that produced by lambda RAL-2 may be useful in attempts to understand the mechanism of O. volvulus-induced ocular pathology.

摘要

一个表达在成虫和幼虫寄生虫中发现的表位的旋盘尾丝虫cDNA克隆，命名为λRAL-2，源自旋盘尾丝虫成虫中存在的一个1000个碱基的信息，该信息编码一种表观分子量为17000的蛋白质。这种蛋白质似乎没有广泛的翻译后修饰。检测了52名暴露于旋盘尾丝虫的个体的血清样本中识别λRAL-2重组抗原的抗体；77%的个体产生了此类抗体。此外，与未产生识别重组抗原抗体的个体相比，产生此类抗体的个体发生与旋盘尾丝虫感染相关的某些眼部病理状况的可能性显著降低。这些结果表明，诸如λRAL-2产生的重组抗原可能有助于理解旋盘尾丝虫诱发眼部病理状况的机制。