Andersen L P, Holck S, Povlsen C O
Dept. of Clinical Microbiology, Statens Seruminstitut, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1988 Jun;96(6):559-64.
An immunohistochemical assay for staining C. pylori is described. The method is compared with cultivation of C. pylori and observation of campylobacter-like organisms (CLOs) in hematoxyline-eosine (HE) stained sections. Eighteen biopsies from which C. pylori was cultivated but not seen in HE stained sections and three culture negative biopsies with CLOs seen in HE stained sections were selected from 331 biopsies including 113 culture positive biopsies. There were agreements between cultivation of C. pylori and CLOs seen in HE stained sections in the remaining 310 biopsies. Fourteen of the 18 and one of the three biopsies were found positive by the immunohistochemical assay. In addition 21 culture-positive control biopsies and one of 18 culture-negative control biopsies were also found positive. When the immunohistochemical assay was compared with cultivation the predictive value of positive result is 93% and of negative result 89%. By this method we were able to detect single organisms and no cross-reactions to other curved bacteria on the gastric epithelium were observed.
本文描述了一种用于幽门螺杆菌染色的免疫组织化学检测方法。该方法与幽门螺杆菌培养法以及苏木精-伊红（HE）染色切片中观察弯曲杆菌样微生物（CLOs）的方法进行了比较。从331份活检组织中选取了18份幽门螺杆菌培养阳性但在HE染色切片中未见的活检组织，以及3份培养阴性但在HE染色切片中可见CLOs的活检组织，其中包括113份培养阳性的活检组织。其余310份活检组织中幽门螺杆菌培养结果与HE染色切片中所见的CLOs结果一致。18份活检组织中的14份以及3份活检组织中的1份通过免疫组织化学检测呈阳性。此外，21份培养阳性对照活检组织以及18份培养阴性对照活检组织中的1份也呈阳性。当将免疫组织化学检测与培养法进行比较时，阳性结果的预测值为93%，阴性结果的预测值为89%。通过这种方法，我们能够检测到单个微生物，并且未观察到胃上皮细胞与其他弯曲菌之间的交叉反应。
