Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Department of Gastroenterology and Hepatology, University Hospital Antwerp, Antwerp University, Edegem, Belgium.
Vet Res. 2023 Oct 17;54(1):93. doi: 10.1186/s13567-023-01223-4.
While seven gastric non-Helicobacter pylori Helicobacter (NHPH) species are known to commonly colonize the stomach of cats and dogs, the potential of H. pylori and H. pylori-like organisms to infect animals remains controversial and was investigated in this study using gastric samples of 20 cats and 27 dogs. A Helicobacter genus-specific 16 S rRNA PCR assay, H. pylori-specific ureAB and glmM PCR assays and a nested PCR detecting 23 S rRNA in a Helicobacter genus-specific manner in a first round of PCR and a H. pylori-specific manner in a second round, were performed in combination with sequencing. Histopathological and anti-Helicobacter immunohistochemical evaluations were also performed. Based on 16 S rRNA sequence analysis, 39/47 animals (83%) appeared infected with canine/feline gastric NHPHs in the corpus and/or antrum. H. pylori-specific ureAB amplicons were obtained in samples of 22 stomachs (47%). One canine antrum sample positive in the ureAB assay was also positive in the H. pylori-specific glmM assay. While 36/47 (77%) animals had a positive sample in the first round of the nested 23 S rRNA PCR assay, all samples were negative in the second round. Sequence analysis of obtained amplicons and immunohistochemistry point towards the presence of unidentified H. pylori-like organisms in cats and dogs. Histopathological examination suggests a low pathogenic significance of the gastric Helicobacter spp. present in these animals. In conclusion, cats and dogs may be (co-)infected with gastric Helicobacter organisms other than the known gastric NHPHs. Culture and isolation should be performed to confirm this hypothesis.
已知七种胃非幽门螺杆菌 Helicobacter（NHPH）物种通常定植于猫和狗的胃中，但幽门螺杆菌和类似幽门螺杆菌的生物体感染动物的潜力仍然存在争议，本研究使用 20 只猫和 27 只狗的胃样本对此进行了调查。采用胃属特异性 16S rRNA PCR 检测、幽门螺杆菌特异性 ureAB 和 glmM PCR 检测以及巢式 PCR 检测，第一轮 PCR 以胃属特异性方式检测 23S rRNA，第二轮 PCR 以幽门螺杆菌特异性方式检测 23S rRNA，并结合测序。还进行了组织病理学和抗幽门螺杆菌免疫组织化学评估。基于 16S rRNA 序列分析，47 只动物中有 39 只（83%）在胃体和/或胃窦中感染了犬/猫胃 NHPH。22 个胃样本中获得了幽门螺杆菌特异性 ureAB 扩增子。ureAB 检测呈阳性的一个犬胃窦样本也在幽门螺杆菌特异性 glmM 检测中呈阳性。虽然第一轮嵌套 23S rRNA PCR 检测中 36/47 只动物（77%）的样本呈阳性，但第二轮所有样本均为阴性。获得的扩增子序列分析和免疫组织化学表明猫和狗中存在未鉴定的幽门螺杆菌样生物体。组织病理学检查表明这些动物胃中存在的幽门螺杆菌 spp. 的致病性较低。总之，猫和狗可能同时感染了除已知胃 NHPH 以外的胃幽门螺杆菌。应进行培养和分离以证实这一假设。
