Regnier M, Desbas C, Bailly C, Darmon M
Centre International de Recherches Dermatologiques (CIRD), Valbonne, France.
In Vitro Cell Dev Biol. 1988 Jul;24(7):625-32. doi: 10.1007/BF02623599.
Normal human keratinocytes isolated from skin and squamous carcinoma cells established from a human tumor (TR146 cell line) both exhibit limited morphologic differentiation when they are grown on conventional plastic dishes. However, when they are seeded on human de-epidermized dermis and cultured at the air-liquid interface, they are able to reform an epithelium having the morphology of the tissue of origin (i.e. skin or squamous carcinoma). The distribution in such reconstructed tissues of differentiation markers such as bullous pemphigoid antigen, 67K keratin, involucrin, membrane-bound transglutaminase, and filaggrin was very similar to their distribution in normal skin and squamous carcinoma specimens, respectively. The degree of differentiation is for both cell types extremely sensitive to culture conditions such as retinoic acid concentration, emersion of the cultures, etc. These results show that subcultured normal or tumoral keratinocytes are able to recover their specific morphogenetic potential when cultured in an environment close to their in vivo situation.
从皮肤分离出的正常人角质形成细胞以及源自人类肿瘤（TR146细胞系）的鳞状癌细胞，当它们在传统塑料培养皿上生长时，均表现出有限的形态分化。然而，当将它们接种到人类去表皮真皮上并在气液界面培养时，它们能够重新形成具有起源组织（即皮肤或鳞状癌）形态的上皮。诸如大疱性类天疱疮抗原、67K角蛋白、兜甲蛋白、膜结合转谷氨酰胺酶和丝聚蛋白等分化标志物在这种重建组织中的分布，分别与它们在正常皮肤和鳞状癌标本中的分布非常相似。对于这两种细胞类型而言，分化程度对诸如视黄酸浓度、培养物暴露等培养条件极为敏感。这些结果表明，传代培养的正常或肿瘤角质形成细胞在接近其体内情况的环境中培养时，能够恢复其特定的形态发生潜能。
