Vasconcelos Mayron Alves, Arruda Francisco Vassiliepe Sousa, Carneiro Victor Alves, Silva Helton Colares, Nascimento Kyria Santiago, Sampaio Alexandre Holanda, Cavada Benildo, Teixeira Edson Holanda, Henriques Mariana, Pereira Maria Olivia
Biologically Active Molecules Laboratory (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60440-970, CE, Brazil ; Institute for Biotechnology and Bioengineering (IBB), Center of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza 60430-160, CE, Brazil.
Biomed Res Int. 2014;2014:365272. doi: 10.1155/2014/365272. Epub 2014 May 28.
This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μ g/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins.
本研究旨在评估植物和藻类凝集素抑制细菌和酵母浮游生长及生物膜形成的能力。最初，以31.25至250μg/mL的浓度对10种凝集素进行了针对表皮葡萄球菌、金黄色葡萄球菌、产酸克雷伯菌、铜绿假单胞菌、白色念珠菌和热带念珠菌的测试。来自多花克拉蒂亚（CFL）、大果瓦泰雷亚（VML）、鲍氏羊蹄甲（BBL）、西弗氏藓藻（BSL）和绳状Hypnea（HML）的凝集素对至少一种微生物表现出活性。还评估了在凝集素存在下生物膜的形成；在用凝集素孵育24小时后，通过量化生物量（用结晶紫染色）和计数活细胞（菌落形成单位）来分析生物膜。根据所测试的微生物不同，凝集素不同程度地降低了生物膜生物量和/或活细胞数量，表明了凝集素的不同特性。这些发现表明，本研究中测试的凝集素可能是天然的替代抗菌剂；然而，需要进一步研究以更好地阐明这些蛋白质的功能用途。
