Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
Separation and Conversion Technologies, VITO - Flemish Institute for Technological Research, Boeretang 200, Mol 2400, Belgium.
Bioresour Technol. 2014 Aug;165:350-4. doi: 10.1016/j.biortech.2014.01.129. Epub 2014 Feb 8.
Bioelectrochemical system (BES) was operated using the enzyme formate dehydrogenase as catalyst at cathode in its free form for the reduction of CO2 into formic acid. Electrosynthesis of formic acid was higher at an operational voltage of -1V vs. Ag/AgCl (9.37mgL(-1) CO2) compared to operation at -0.8V (4.73mgL(-1) CO2) which was strongly supported by the reduction catalytic current. Voltammograms also depicted a reversible redox peak throughout operation at -1V, indicating NAD(+) recycling for proton transfer from the source to CO2. Saturation of the product was observed after 45min of enzyme addition and then reversibility commenced, depicting a lower and stable formic acid concentration throughout the subsequent time of operation. Stability of the enzyme activity after immobilization on the electrode and product yield will be studied further.
生物电化学系统（BES）在其游离形式的阴极上使用甲酸脱氢酶作为催化剂运行，用于将 CO2 还原为甲酸。与在-0.8V（4.73mgL(-1) CO2）下操作相比，在-1V 下操作时甲酸的电合成更高，这得到了还原催化电流的有力支持。循环伏安图还描绘了在-1V 下整个操作过程中的可逆氧化还原峰，表明 NAD(+) 为质子从源转移到 CO2 进行循环。在添加酶 45 分钟后观察到产物饱和，然后开始可逆性，在随后的操作时间内，甲酸浓度较低且稳定。将酶固定在电极上后的酶活性稳定性和产物产率将进一步研究。
