CNRS, Aix-Marseille Université, BIP, IMM, IM2B, 31 Chemin J. Aiguier, CS70071, 13402, Marseille Cedex 20, France.
Instituto de Tecnologia Química e Biológica António Xavier (ITQB NOVA), Universidade Nova de Lisboa, Oeiras, Portugal.
Angew Chem Int Ed Engl. 2021 Apr 26;60(18):9964-9967. doi: 10.1002/anie.202101167. Epub 2021 Mar 18.
Mo/W formate dehydrogenases catalyze the reversible reduction of CO species to formate. It is thought that the substrate is CO and not a hydrated species like HCO , but there is still no indisputable evidence for this, in spite of the extreme importance of the nature of the substrate for mechanistic studies. We devised a simple electrochemical method to definitively demonstrate that the substrate of formate dehydrogenases is indeed CO .
钼/钨甲酸盐脱氢酶催化 CO 物种的可逆还原生成甲酸盐。人们认为底物是 CO，而不是 HCO 等水合物种，但尽管底物的性质对于机理研究至关重要，但仍没有确凿的证据支持这一点。我们设计了一种简单的电化学方法来明确证明甲酸盐脱氢酶的底物确实是 CO。
