Magalon Jeremy, Bausset Olivier, Veran Julie, Giraudo Laurent, Serratrice Nicolas, Magalon Guy, Dignat-George Françoise, Sabatier Florence
Culture and Therapy Unit, CHU La Conception (AP-HM) , Marseilles, France .
Department of Plastic & Reconstructive Surgery, CHU La Conception (AP-HM) , Marseilles, France .
Biores Open Access. 2014 Feb 1;3(1):35-8. doi: 10.1089/biores.2013.0041.
Autologous conditioned serum (ACS) is a recent biotherapy based on certain cytokines anti-inflammatory properties mainly used for the reduction of osteoarthritis (OA) symptoms. Here we investigated different physico-chemical factors influencing ACS purification and cytokine production. Human venous blood was incubated in the presence of different diameter beads (respectively 2.5, 3, 3.5, and 4 mm) or glass beads with different types of coating (polished or coated with CrSO4). Sera were recovered, and the concentrations of pro-inflammatory and anti-inflammatory relevant cytokines were measured using Luminex(®) technology. Fresh whole blood incubated for 24 h highly increased production of interleukin (IL)-6 and IL-8 cytokines. At the same time, the concentrations of IL-1β, IL-1 receptor agonist (IL-1Ra), IL-10, and tumor necrosis factor (TNF)-α were slightly induced. The highest cytokine concentrations were obtained with the exposure of whole blood to 3-mm glass beads and 3.5-mm polished beads. The minimum IL-1β/IL-1Ra ratio obtained was 3.2±1.3 after 24-h incubation without any beads. ACS has been shown to alleviate clinical symptoms of OA in clinical studies. This descriptive study demonstrated that different pro- and anti-inflammatory cytokines are present in ACS since no selective anti-inflammatory cytokines were produced based on the different protocols. Furthermore, we showed that CrSO4-treated glass beads are not necessary and that the absence of beads combined with a 24-h incubation could also lead to an enriched serum.
自体条件血清（ACS）是一种基于某些细胞因子抗炎特性的新型生物疗法，主要用于减轻骨关节炎（OA）症状。在此，我们研究了影响ACS纯化和细胞因子产生的不同物理化学因素。将人静脉血在不同直径的珠子（分别为2.5、3、3.5和4毫米）或具有不同类型涂层（抛光或涂有硫酸铬）的玻璃珠存在下孵育。收集血清，并使用Luminex（®）技术测量促炎和抗炎相关细胞因子的浓度。孵育24小时的新鲜全血可显著增加白细胞介素（IL）-6和IL-8细胞因子的产生。同时，IL-1β、IL-1受体激动剂（IL-1Ra）、IL-10和肿瘤坏死因子（TNF）-α的浓度略有升高。全血暴露于3毫米玻璃珠和3.5毫米抛光珠时可获得最高的细胞因子浓度。在无任何珠子孵育24小时后，获得的最低IL-1β/IL-1Ra比值为3.2±1.3。临床研究表明，ACS可缓解OA的临床症状。这项描述性研究表明，ACS中存在不同的促炎和抗炎细胞因子，因为根据不同方案未产生选择性抗炎细胞因子。此外，我们表明硫酸铬处理的玻璃珠并非必需，无珠子结合24小时孵育也可产生富集血清。
