Meijer H, Reinecke J, Becker C, Tholen G, Wehling P
ORTHOGEN Lab Services, Graf-Adolf-Strasse 43, 40210 Düsseldorf, Germany.
Inflamm Res. 2003 Oct;52(10):404-7. doi: 10.1007/s00011-003-1197-1.
Cytokines such as interleukin-1 (IL-1) play an important role in degenerative musculo-skeletal diseases, including osteoarthritis, and a multitude of inflammatory disorders. Agents that inhibit the action of such cytokines have a high therapeutic potential in such diseases. Here we describe a new method for enhancing the production of the interleukin-l receptor antagonist (IL-1Ra) and other anti-inflammatory cytokines in whole blood.
Human venous blood was incubated in the presence of CrSO(4)-treated glass beads. Serum was recovered and the concentrations of IL-1Ra and other relevant cytokines were measured by ELISA.
The interaction of the glass bead surface with cells in whole blood increased production of IL-1Ra and anti-inflammatory cytokines. Removal of the beads and centrifugation generated a serum preparation enriched in anti-inflammatory cytokines. This preparation is of therapeutic value in treating various inflammatory and degenerative disorders.
The increased de novo production of anti-inflammatory cytokines by a direct physico-chemical induction of whole blood in the Orthokin system is feasible and offers an alternative, novel approach to treating mild to moderate OA and other orthopaedic conditions such as degenerative spine diseases.
细胞因子如白细胞介素-1（IL-1）在包括骨关节炎在内的退行性肌肉骨骼疾病以及多种炎症性疾病中发挥重要作用。抑制此类细胞因子作用的药物在这些疾病中具有很高的治疗潜力。在此，我们描述了一种增强全血中白细胞介素-1受体拮抗剂（IL-1Ra）和其他抗炎细胞因子产生的新方法。
将人静脉血在经硫酸铬（CrSO₄）处理的玻璃珠存在下孵育。回收血清，并通过酶联免疫吸附测定法（ELISA）测量IL-1Ra和其他相关细胞因子的浓度。
玻璃珠表面与全血细胞的相互作用增加了IL-1Ra和抗炎细胞因子的产生。去除珠子并离心后得到了富含抗炎细胞因子的血清制剂。该制剂在治疗各种炎症性和退行性疾病方面具有治疗价值。
在正位运动系统中通过对全血进行直接物理化学诱导来增加抗炎细胞因子的从头产生是可行的，并且为治疗轻度至中度骨关节炎和其他骨科疾病（如退行性脊柱疾病）提供了一种替代的新方法。
