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使用单丹磺酰戊烷作为蓝色荧光脂滴标记物，实现多色活细胞成像。

Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging.

机构信息

Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei, Taiwan.

出版信息

PLoS One. 2012;7(3):e32693. doi: 10.1371/journal.pone.0032693. Epub 2012 Mar 1.

DOI:10.1371/journal.pone.0032693
PMID:22396789
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3291611/
Abstract

Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.

摘要

脂滴（LDs）是负责储存中性脂质的动态细胞细胞器，与多种代谢综合征有关。在这里，我们报告了 1,1'-二甲基-4,4'-联苯二酰亚胺（MDH）作为 LD 的高对比度蓝色荧光标记物。独特的光谱特性使 MDH 可以轻松与其他绿色和红色荧光报告分子结合，用于多色荧光成像。MDH 染色不会明显影响 LD 的运输，并且该染料具有极高的光稳定性。总之，MDH 是一种可靠的工具，可用于使用荧光显微镜研究活细胞内动态 LD 调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/bef789e3b89b/pone.0032693.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/ba9e9ac8fe06/pone.0032693.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/6053295ba6b2/pone.0032693.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/5f5badfaea29/pone.0032693.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/4ad32ab3fb26/pone.0032693.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/e7f359f97261/pone.0032693.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/bef789e3b89b/pone.0032693.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/ba9e9ac8fe06/pone.0032693.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/6053295ba6b2/pone.0032693.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/5f5badfaea29/pone.0032693.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/4ad32ab3fb26/pone.0032693.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/e7f359f97261/pone.0032693.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7afb/3291611/bef789e3b89b/pone.0032693.g006.jpg

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