Kasper M, Moll R, Stosiek P, Karsten U
Institute of Pathology, District Hospital Görlitz, German Democratic Republic.
Histochemistry. 1988;89(4):369-77. doi: 10.1007/BF00500639.
We studied the expression of the various cytokeratin (CK) polypeptides and vimentin in tissues of the human eye by applying immunocytochemical procedures using a panel of monoclonal antibodies as well as by performing biochemical analyses of microdissected tissues. Adult corneal epithelium was found to contain significant amounts of the cornea-specific CKs nos. 3 and 12 as well as CK no. 5, and several additional minor CK components. Among these last CKs, no. 19 was found to exhibit an irregular mosaic-like staining pattern in the peripheral zone of the corneal epithelium, while having a predominantly basal distribution in the limbal epithelium. Both the fetal corneal epithelium and the conjunctival epithelium were uniformly positive for CK no. 19. In the ciliary epithelium, co-expression of CKs nos. 8 and 18 and vimentin was detected, whereas in the retinal pigment epithelium, CKs nos. 8 and 18 were dominant. The present data illustrate the remarkable diversity and complexity of CK-polypeptide expression in the human eye, whose significance with respect to histogenetic and functional aspects is, as yet, only partially clear. The unusual distribution of CK no. 19 in different zones of the corneal epithelium may be related to the specific topography of corneal stem cells. The occurrence of the expression of simple-epithelium CKs in the ciliary and pigment epithelium demonstrates that, despite their neuroectodermal derivation, these are true epithelia.
我们通过应用一组单克隆抗体的免疫细胞化学方法以及对显微切割组织进行生化分析，研究了人眼组织中各种细胞角蛋白（CK）多肽和波形蛋白的表达情况。发现成人角膜上皮含有大量角膜特异性CK3和CK12以及CK5，还有几种其他次要的CK成分。在这些最后的CK中，CK19在角膜上皮的周边区域呈现不规则的马赛克样染色模式，而在角膜缘上皮中主要分布在基底。胎儿角膜上皮和结膜上皮对CK19均呈均匀阳性。在睫状体上皮中，检测到CK8和CK18与波形蛋白的共表达，而在视网膜色素上皮中，CK8和CK18占主导。目前的数据说明了人眼中CK多肽表达的显著多样性和复杂性，其在组织发生和功能方面的意义目前仅部分明确。CK19在角膜上皮不同区域的异常分布可能与角膜干细胞的特定拓扑结构有关。在睫状体和色素上皮中出现简单上皮CK的表达表明，尽管它们起源于神经外胚层，但这些都是真正的上皮。
