在肾肿瘤和睾丸肿瘤中未检测到赭曲霉毒素A。

Ochratoxin A is not detectable in renal and testicular tumours.

作者信息

Fahmy Nader, Woo Mark, Alameldin Mona, Macdonald Kyle, Goneau Lee W, Cadieux Peter, Pautler Stephen E

机构信息

Division of Urology, Department of Surgery, Schulich School of Medicine & Dentistry, Western University, London, ON.

Department of Pathology, Schulich School of Medicine & Dentistry, Western University, London, ON;

出版信息

Can Urol Assoc J. 2014 Jan-Feb;8(1-2):40-6. doi: 10.5489/cuaj.1240.

DOI:10.5489/cuaj.1240

PMID:24578744

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3929480/

Abstract

INTRODUCTION

Ochratoxin-A (OTA) is one of the most abundant food-contaminating mycotoxins, known for its nephrotoxicity, neurotoxicity, gonadotoxicity, teratogenicity, immunosuppression and carcinogenesis. OTA has been linked to several genitourinary pathologies, including Balkan nephropathy and genitourinary malignancies. We examine OTA levels in serum samples and tumour specimens collected from patients with renal and testicular tumours.

METHODS

Frozen samples were obtained from the Ontario Tumour Bank. Serum specimens, along with renal and testicular tumour biopsies, were included in this study. Normal tissue from the negative surgical margins of each tumour served as a control. OTA levels in serum was measured using the enzyme-linked immunosorbent assay (ELISA), while OTA detection in tissue specimens was determined using immunohistochemistry (IHC).

RESULTS

We included specimens collected from 56 patients (36 men and 20 women). Histopathology of the 52 renal tumours included 31 (60%) conventional type renal cell carcinomas (RCC), 5 (10%) chromophobe RCC, 5 (10%) papillary RCC, 1 (2%) oncocytoma and 10 (19%) upper tract urothelial carcinoma (UC). The 4 testicular tumours included 1 seminomatous (25%) germ cell tumour and 3 (75%) non-seminomatous germ cell tumours. OTA was detected in the serum of renal tumour patients, with a range from 0.004 to 0.25 ng/mL (mean: 0.07 and median 0.06 ng/mL). There was no OTA signal detected by IHC staining in all tested renal and testicular tumours.

CONCLUSIONS

The OTA levels detected in the serum of patients were highly variable and relatively low. No OTA was detected in the tissue samples.

摘要

引言

赭曲霉毒素A（OTA）是最常见的污染食品的霉菌毒素之一，以其肾毒性、神经毒性、性腺毒性、致畸性、免疫抑制和致癌作用而闻名。OTA与多种泌尿生殖系统疾病有关，包括巴尔干肾病和泌尿生殖系统恶性肿瘤。我们检测了从肾肿瘤和睾丸肿瘤患者采集的血清样本和肿瘤标本中的OTA水平。

方法

从安大略肿瘤库获得冷冻样本。本研究纳入了血清标本以及肾和睾丸肿瘤活检样本。每个肿瘤阴性手术切缘的正常组织作为对照。血清中的OTA水平采用酶联免疫吸附测定（ELISA）进行检测，而组织标本中的OTA检测则采用免疫组织化学（IHC）方法。

结果

我们纳入了56例患者（36例男性和20例女性）的标本。52例肾肿瘤的组织病理学检查包括31例（60%）传统型肾细胞癌（RCC）、5例（10%）嫌色性RCC、5例（10%）乳头状RCC、1例（2%）嗜酸细胞瘤和10例（19%）上尿路尿路上皮癌（UC）。4例睾丸肿瘤包括1例（25%）精原细胞瘤性生殖细胞肿瘤和3例（75%）非精原细胞瘤性生殖细胞肿瘤。在肾肿瘤患者的血清中检测到OTA，范围为0.004至0.25 ng/mL（平均值：0.07，中位数0.06 ng/mL）。在所有检测的肾和睾丸肿瘤中，免疫组织化学染色均未检测到OTA信号。