Suwabe A, Takahashi K, Yasui S, Arai S, Sendo F
First Department of Intenral Medicine, Yamagata University School of Medicine, Japan.
Am J Pathol. 1988 Sep;132(3):512-20.
The capacity of alveolar macrophages (AM) of bleomycin-instilled hamsters to proliferate mouse thymocytes (interleukin-1 activity) and hamster fibroblasts (fibroblast proliferation (FP) activity was studied. Using bleomycin-instilled hamsters, the FP activity of AM culture supernatants was increased significantly on days 1, 5, and 10 after instillation of bleomycin. The interleukin-1 (IL-1) activity, however, was increased significantly on day 1 only as compared with saline-treated hamsters. Next, normal AM were stimulated in vitro by bleomycin. After being fractionated by chromatography, their culture supernatant showed IL-1 activity, which also indicated FP activity. These results suggest that bleomycin directly stimulates AM to release IL-1 in the fibrogenic responses.
研究了博来霉素注入仓鼠后肺泡巨噬细胞(AM)增殖小鼠胸腺细胞的能力(白细胞介素-1活性)以及仓鼠成纤维细胞(成纤维细胞增殖(FP)活性)。使用博来霉素注入的仓鼠,在注入博来霉素后的第1、5和10天,AM培养上清液的FP活性显著增加。然而,与生理盐水处理的仓鼠相比,白细胞介素-1(IL-1)活性仅在第1天显著增加。接下来,用博来霉素体外刺激正常AM。经色谱分离后,其培养上清液显示出IL-1活性,这也表明存在FP活性。这些结果表明,在纤维化反应中,博来霉素直接刺激AM释放IL-1。