Fiore M, Mitchell J, Doan T, Nelson R, Winter G, Grandone C, Zeng K, Haraden R, Smith J, Harris K
Abbott Laboratories, N. Chicago, IL 60064.
Clin Chem. 1988 Sep;34(9):1726-32.
We describe a new clinical laboratory instrument, the IMx, used to automate immunoassay testing in the clinical laboratory. The IMx incorporates a novel technology called Microparticle capture Enzyme ImmunoAssay (MEIA) for assays of high-molecular-mass analytes, and fluorescence polarization immunoassay (FPIA) for hapten assays. A front-surface fluorometer is used to quantify the enzymatic generation of fluorescent product at a rate proportional to the concentration of the analyte in an MEIA, and a fluorescence polarization optical system is used to quantify results in an FPIA. The microprocessor-based instrument uses a robotic arm with two degrees of freedom and a rotating carousel to process the samples for assay. One assay can be done on each of 24 patients' specimens in 30 to 40 min with "walk-away" automation. Calibration curves are stable for at least two weeks. Instrument control involves software-labeled "command keys," a numeric keypad, and an interactive display. Results are output to a thermal printer or computer interface.
我们介绍一种新的临床实验室仪器——IMx，用于临床实验室免疫分析检测的自动化。IMx采用了一种名为微粒捕获酶免疫分析（MEIA）的新技术来检测高分子量分析物，以及荧光偏振免疫分析（FPIA）来进行半抗原分析。前表面荧光计用于以与MEIA中分析物浓度成比例的速率定量荧光产物的酶促生成，荧光偏振光学系统用于定量FPIA中的结果。基于微处理器的仪器使用具有两个自由度的机械臂和旋转圆盘传送装置来处理待分析的样本。通过“无需人工干预”的自动化操作，在30至40分钟内可对24位患者的每份标本进行一次检测。校准曲线至少两周内保持稳定。仪器控制包括标有软件的“命令键”、数字小键盘和交互式显示屏。结果输出至热敏打印机或计算机接口。
