Anyasor Godswill Nduka, Onajobi Funmilayo, Osilesi Odutola, Adebawo Olugbenga, Oboutor Efere Martins
Department of Biochemistry, Benjamin S. Carson School of Medicine, College of Health and Medical Sciences, Babcock University, Ilisan Remo, Ogun State, P.M.B. 21244 Ikeja, Lagos, Nigeria.
Department of Biochemistry, Benjamin S. Carson School of Medicine, College of Health and Medical Sciences, Babcock University, Ilisan Remo, Ogun State, P.M.B. 21244 Ikeja, Lagos, Nigeria.
J Ethnopharmacol. 2014 Aug 8;155(1):543-51. doi: 10.1016/j.jep.2014.05.057. Epub 2014 Jun 6.
Costus afer Ker Gawl is an indigenous tropical African medicinal plant used as therapy in the treatment of inflammatory ailments such as rheumatoid arthritis. This study was designed to evaluate the anti-inflammatory and antioxidant activities of the hexane fraction of C. afer leaves (CAHLF).
The anti-inflammatory effect of varying doses of CAHLF on carrageenan, arachidonic acid, and formaldehyde induced arthritis in male albino rats׳ models were investigated in order to study the acute inflammatory phase. Complete Freund׳s Adjuvant (CFA)-induced arthritis model was used to study the chronic inflammatory phase. Two known anti-inflammatory drugs, Diclofenac sodium (non-steroidal anti-inflammatory drug [NSAID]) and prednisolone (glucocorticoid [steroidal drug]) were used as standards for comparison. Various biochemical indices viz. superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), reduced glutathione (GSH) and malondialdehyde (MDA), aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), total protein (TP), globulin and albumin levels were assayed using spectrophotometric methods.
Control animals in which arthritis have been induced using carrageenan, arachidonic acid, formaldehyde or CFA showed significant increases (P<0.05) in paw edema when compared with normal animals. Treatment of the arthritis induced rats with CAHLF significantly (P<0.05) suppressed the edema. in vivo antioxidant study showed that CAHLF treated animals had a significantly (P<0.05) elevated GSH level, SOD, CAT and GST activities while MDA levels were significantly (P<0.05) reduced in the plasma, liver, kidney and brain. CAHLF treated rats had a significantly (P<0.05) reduced plasma AST, ALT and ALP. Plasma TP, globulin, TB levels were reduced while albumin levels were elevated in CAHLF treated animals.
CAHLF possesses substantial anti-inflammatory and antioxidant activities against inflammatory diseases especially arthritis. It could be considered as a choice candidate in pharmaceutical anti-inflammatory drug development.
非洲刺毛姜是一种原产于热带非洲的药用植物，被用于治疗类风湿性关节炎等炎症性疾病。本研究旨在评估非洲刺毛姜叶己烷提取物（CAHLF）的抗炎和抗氧化活性。
为研究急性期炎症，考察了不同剂量的CAHLF对雄性白化大鼠角叉菜胶、花生四烯酸和甲醛诱导的关节炎的抗炎作用。采用完全弗氏佐剂（CFA）诱导的关节炎模型研究慢性炎症期。两种已知的抗炎药物双氯芬酸钠（非甾体抗炎药[NSAID]）和泼尼松龙（糖皮质激素[甾体药物]）用作比较标准。使用分光光度法测定各种生化指标，即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）、还原型谷胱甘肽（GSH）和丙二醛（MDA）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、总胆红素（TB）、总蛋白（TP）、球蛋白和白蛋白水平。
与正常动物相比，用角叉菜胶、花生四烯酸、甲醛或CFA诱导关节炎的对照动物爪部水肿显著增加（P<0.05）。用CAHLF治疗关节炎诱导大鼠可显著（P<0.05）抑制水肿。体内抗氧化研究表明，CAHLF处理的动物血浆、肝脏、肾脏和大脑中的GSH水平、SOD、CAT和GST活性显著（P<0.05）升高，而MDA水平显著（P<0.05）降低。CAHLF处理的大鼠血浆AST、ALT和ALP显著（P<0.05）降低。CAHLF处理的动物血浆TP、球蛋白、TB水平降低，而白蛋白水平升高。
CAHLF对炎症性疾病尤其是关节炎具有显著的抗炎和抗氧化活性。它可被视为药物抗炎药物开发的候选选择。
