Wang Xiaochun, Shao Shihe, Wang Hua, Shen Quan, Yang Shixing, Zhang Wen
Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Zhenjiang, 212013 Jiangsu China.
Springerplus. 2016 Aug 8;5(1):1281. doi: 10.1186/s40064-016-2966-y. eCollection 2016.
Enteroviruses (EVs) are a genetically and antigenically diverse group of viruses infecting humans and a variety of animals including non-human primates (NHPs). The present study was to investigate EVs in the fecal samples from captive NHPs in zoos in China using classic RT-PCR and viral metagenomics methods.
An EV strain was detected in a fecal sample collected from a captive NHP of a zoo in eastern China. The complete genome of this EV strain (named Sev-nj1) was determined and characterized. Sequence analysis indicated Sev-nj1 shared the highest sequence identity (75.6 %) with an EV-J strain, Poo-1, based on the complete genome. Phylogenetic analysis showed Sev-nj1 clustered with the other EV-J strains, forming a separate clade.
According to the genetic distance-based criteria, Sev-nj1 belonged to a new type within the species EV-J. This is the first study detecting EV-J from a NHP in China, which will be helpful for the future epidemiology study of EVs in NHPs.
肠道病毒（EVs）是一类在基因和抗原性上具有多样性的病毒，可感染人类以及包括非人灵长类动物（NHPs）在内的多种动物。本研究旨在运用经典逆转录聚合酶链反应（RT-PCR）和病毒宏基因组学方法，对中国动物园圈养非人灵长类动物粪便样本中的肠道病毒进行调查。
在中国东部一家动物园的圈养非人灵长类动物粪便样本中检测到一株肠道病毒。测定并鉴定了该肠道病毒株（命名为Sev-nj1）的全基因组。序列分析表明，基于全基因组，Sev-nj1与一株肠道病毒J型毒株Poo-1具有最高的序列同一性（75.6%）。系统发育分析显示，Sev-nj1与其他肠道病毒J型毒株聚类，形成一个独立的进化分支。
根据基于遗传距离的标准，Sev-nj1属于肠道病毒J型物种内的一个新型。这是中国首次从非人灵长类动物中检测到肠道病毒J型，将有助于未来对非人灵长类动物肠道病毒的流行病学研究。
