Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, People's Republic of China.
DNA Cell Biol. 2012 May;31(5):761-5. doi: 10.1089/dna.2011.1326. Epub 2011 Oct 19.
MicroRNAs are noncoding RNA molecules of 18-25 nucleotides that regulate gene expression at the post-transcriptional level. The aim of this study was to investigate whether pri-miR-218 rs11134527 A/G polymorphism influences the risk of hepatocellular carcinoma (HCC) or not. pri-miR-218 rs11134527 A/G was genotyped in 302 HCC patients and 513 control subjects using the polymerase chain reaction-restriction fragment length polymorphism assay. The AG genotype of pri-miR-218 rs11134527 A/G was associated with family history (p=0.018, odds ratio [OR]=2.96, 95% confidence interval [CI]: 1.16-7.56) and elevated serum α-fetoprotein (serum alpha-fetoprotein [AFP]) levels (≥20 ng/mL; p=0.009, OR=1.92, 95% CI: 1.17-3.14) in HCC patients. These findings suggested that the AG genotype of pri-miR-218 rs11134527 might relate to genetic predisposition and be involved in regulating the expression of AFP in Chinese HCC patients.
微小 RNA 是 18-25 个核苷酸的非编码 RNA 分子，可在转录后水平调节基因表达。本研究旨在探讨 pri-miR-218 rs11134527 A/G 多态性是否影响肝细胞癌 (HCC) 的发病风险。采用聚合酶链反应-限制性片段长度多态性分析方法，对 302 例 HCC 患者和 513 例对照进行了 pri-miR-218 rs11134527 A/G 基因分型。pri-miR-218 rs11134527 A/G 的 AG 基因型与家族史有关（p=0.018，比值比 [OR]=2.96，95%置信区间 [CI]：1.16-7.56），与 HCC 患者血清 α-胎蛋白（血清甲胎蛋白 [AFP]）水平升高（≥20ng/ml;p=0.009，OR=1.92，95% CI：1.17-3.14）有关。这些发现表明，pri-miR-218 rs11134527 的 AG 基因型可能与遗传易感性有关，并参与调节中国 HCC 患者 AFP 的表达。
