Vanyushkina Anna A, Fisunov Gleb Y, Gorbachev Alexey Y, Kamashev Dmitri E, Govorun Vadim M
Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation.
Russian Institute of Physico-Chemical Medicine, Moscow, Russian Federation; Russian Research Center Kurchatov Institute, Moscow, Russian Federation.
PLoS One. 2014 Mar 4;9(3):e89312. doi: 10.1371/journal.pone.0089312. eCollection 2014.
We present a systematic study of three bacterial species that belong to the class Mollicutes, the smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, and Acholeplasma laidlawii. To understand the difference in the basic principles of metabolism regulation and adaptation to environmental conditions in the three species, we analyzed the metabolome of these bacteria. Metabolic pathways were reconstructed using the proteogenomic annotation data provided by our lab. The results of metabolome, proteome and genome profiling suggest a fundamental difference in the adaptation of the three closely related Mollicute species to stress conditions. As the transaldolase is not annotated in Mollicutes, we propose variants of the pentose phosphate pathway catalyzed by annotated enzymes for three species. For metabolite detection we employed high performance liquid chromatography coupled with mass spectrometry. We used liquid chromatography method - hydrophilic interaction chromatography with silica column - as it effectively separates highly polar cellular metabolites prior to their detection by mass spectrometer.
我们对属于柔膜菌纲的三种细菌进行了系统研究，柔膜菌纲是最小且最简单的细菌，包括蜜蜂螺旋体、鸡败血支原体和解脲脲原体。为了解这三种细菌在代谢调控基本原理以及对环境条件适应性方面的差异，我们分析了这些细菌的代谢组。利用我们实验室提供的蛋白质基因组注释数据重建了代谢途径。代谢组、蛋白质组和基因组分析结果表明，这三种密切相关的柔膜菌属细菌在适应应激条件方面存在根本差异。由于在柔膜菌纲中未注释转醛醇酶，我们提出了由已注释酶催化的三种细菌磷酸戊糖途径的变体。对于代谢物检测，我们采用了高效液相色谱与质谱联用技术。我们使用液相色谱法——硅胶柱亲水相互作用色谱法——因为它能在通过质谱仪检测之前有效分离高极性细胞代谢物。
