Hedreen J C, Raskin L S, Struble R G, Price D L
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD.
J Neurosci Methods. 1988 Sep;25(2):151-8. doi: 10.1016/0165-0270(88)90153-7.
Most silver methods for the demonstration of senile plaques (SP) in Alzheimer's disease (AD) give variable staining of SP and often stain a variety of other structures (including normal axons, neurofibrillary tangles (NFT), blood vessel reticulin, nucleoli of neurons, and nuclei of glia). A newly developed silver method for paraffin sections is described in this report that reliably stains densely arrayed fine processes in SP. Other structures, including NFT, are not darkly stained. The high-contrast staining of SP greatly facilitates the mapping and morphometry of SP using videomicroscopic image analysis systems and provides a new method for other research studies on SP and for diagnostic histopathology.
大多数用于在阿尔茨海默病（AD）中显示老年斑（SP）的银染方法对SP的染色效果不一，且常常会使多种其他结构（包括正常轴突、神经原纤维缠结（NFT）、血管网状纤维、神经元核仁和神经胶质细胞核）着色。本报告描述了一种新开发的用于石蜡切片的银染方法，该方法能可靠地染出SP中密集排列的细纤维。包括NFT在内的其他结构不会被深染。SP的高对比度染色极大地促进了使用视频显微镜图像分析系统对SP进行定位和形态测量，并为关于SP的其他研究以及诊断组织病理学提供了一种新方法。
