Connolly A A, Anderton B A, Esiri M M
Department of Neuropathology, Radcliffe Infirmary, Oxford, UK.
J Neurol Neurosurg Psychiatry. 1987 Sep;50(9):1221-4. doi: 10.1136/jnnp.50.9.1221.
Serial sections of brains of eight cases with Alzheimer's disease were stained by methods utilising silver and three different monoclonal antibodies. The numbers of Alzheimer's neurofibrillary tangles (NFT) were counted in the silver stained sections and compared with the numbers detected by three monoclonal anti-NF antibodies: RT97, BF10 and 8D8. The monoclonal antibody (Mab) BF10 demonstrated NFTs more clearly than Mab 8D8, but neither detected as many tangles as the silver staining method. Mab RT97 reacted well with NFTs in freshly processed specimens but not following prolonged fixation.
对8例阿尔茨海默病患者的大脑连续切片采用银染法及三种不同的单克隆抗体进行染色。在银染切片中计数阿尔茨海默病神经原纤维缠结（NFT）的数量，并与三种单克隆抗神经丝抗体（RT97、BF10和8D8）检测到的数量进行比较。单克隆抗体（Mab）BF10比Mab 8D8更清晰地显示NFT，但两者检测到的缠结数量均不如银染法多。Mab RT97在新鲜处理的标本中与NFT反应良好，但在长时间固定后则不然。
