MANA, National Institute for Materials Science, 1-1 Namiki, Tsukuba-city, Ibaraki, 305-0044 (Japan).
Angew Chem Int Ed Engl. 2014 Apr 7;53(15):3993-5. doi: 10.1002/anie.201311192. Epub 2014 Mar 5.
The nicotinamide adenine dinucleotide (NAD) derivatives NADH and NADPH are critical components of cellular energy metabolism and operate as electron carriers. A novel fluorescent ubiquinone-rhodol derivative (UQ-Rh) was developed as a probe for NAD(P)H. By using the artificial promoter (η(5) -C5 Me5 )Ir(phen)(H2 O) , intracellular activation and imaging of NAD(P)H were successfully demonstrated. In contrast to bioorthogonal chemistry, this "bioparallel chemistry" approach involves interactions with native biological processes and could potentially be used to control or investigate cellular systems.
烟酰胺腺嘌呤二核苷酸 (NAD) 衍生物 NADH 和 NADPH 是细胞能量代谢的关键组成部分，它们作为电子载体发挥作用。一种新型的荧光泛醌-罗丹明衍生物 (UQ-Rh) 被开发为 NAD(P)H 的探针。通过使用人工启动子 (η(5) -C5 Me5 )Ir(phen)(H2 O) ，成功地实现了 NAD(P)H 的细胞内激活和成像。与生物正交化学相比，这种“生物平行化学”方法涉及与天然生物过程的相互作用，并且可能用于控制或研究细胞系统。
