Lin S C, Lin Y H, Chen C F, Chung C Y, Hsu S H
Department of Pharmacology, Taipei Medical College, Taiwan.
Am J Chin Med. 1997;25(3-4):325-32. doi: 10.1142/S0192415X97000366.
Propolis designates a mixture of gums, resins and balms, of viscous consistency, which are gathered on certain parts (buds and bark, mainly) of vegetables (especially coniferous trees) by honeybees. They bring this back to the hive, where it is modified and mixed with other substances (essentially their own wax and salivary secretions). In this study, the hepatoprotective and therapeutic effects of propolis ethanol extract on chronic alcohol-induced liver injuries were investigated in rats. 3.125 ml of 99.5% alcohol was added to animal's daily diet for four weeks to induce chronic alcohol liver injuries. After sacrifice, serum transaminases (GOT, GPT), triacylglyceride and hepatic triacylglyceride (HTG) concentration were assayed to observe liver injuries induced by chronic alcohol abuse. In addition, the phenomenon of alcohol induced fatty liver were also observed by histopathological changes. Different doses of propolis ethanol extract were p.o. administered three times per day for three days, after four weeks' alcohol administration. It was found that 10 mg/kg of propolis ethanol extract significantly decreased the elevations of serum GOT, GPT, TG and HTG. In histopathological examination, 30 mg/kg of propolis ethanol extract also remarkably decreased the hepatocellular fatty degeneration, apparent as vacuolization, induced by chronic alcohol abuse.
蜂胶是一种由树胶、树脂和香脂混合而成的物质，质地粘稠，由蜜蜂采集自蔬菜（尤其是针叶树）的某些部位（主要是芽和树皮）。它们将其带回蜂巢，在那里进行加工并与其他物质（主要是它们自身的蜡和唾液分泌物）混合。在本研究中，对大鼠慢性酒精性肝损伤模型，研究了蜂胶乙醇提取物的保肝及治疗作用。在动物的日常饮食中添加3.125 ml 99.5%的酒精，持续四周以诱导慢性酒精性肝损伤。处死动物后，检测血清转氨酶（谷草转氨酶、谷丙转氨酶）、甘油三酯和肝脏甘油三酯（HTG）浓度，以观察慢性酒精滥用所致的肝损伤。此外，还通过组织病理学变化观察酒精性脂肪肝现象。在给予四周酒精后，每天口服不同剂量的蜂胶乙醇提取物，连续三天，每天三次。结果发现，10 mg/kg的蜂胶乙醇提取物能显著降低血清谷草转氨酶、谷丙转氨酶、甘油三酯和肝脏甘油三酯的升高。在组织病理学检查中，30 mg/kg的蜂胶乙醇提取物也能显著减轻慢性酒精滥用所致的肝细胞脂肪变性，表现为空泡化。
